Anti bcl 2 c 2

Antibodies were obtained from the following sources; mouse anti-Nestin antibody (BD Transduction Laboratories), mouse anti-Synaptophysin (7H12, Cell Signaling Technology), rabbit anti-Map2 (D5G1, Cell Signaling Technology), anti-Cleaved Caspase-3 (Ab-2, Millipore-Sigma Aldrich), anti-PUMAα/β Antibody (G-3, Santa Cruz Biotechnology, Inc.), anti-Bcl-2 (C-2, Santa Cruz Biotechnology, Inc.), anti-Mcl-1 (S-19, Santa Cruz Biotechnology, Inc.), anti-Bax (N-20, Santa Cruz Biotechnology, Inc.), anti-Bad (Cell Signaling Technology), anti-SRSF1 (3G268, Santa Cruz Biotechnology, Inc.), anti-Tubulin (Millipore-Sigma-Aldrich). Mammalian protease inhibitors were obtained from Sigma-Aldrich (St Louis, MO). Bradford reagent was from BioWorld (Dublin, OH). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) was obtained from Thermo Fisher Scientific (#M6494).

MV4-11 cells were treated for four hours with 10 nM Venetoclax, 5 nM S63845 or the drug combination. Cell lysates were prepared, separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. Detection antibodies included anti-MCL1 (S-19, sc-819) and anti-Bcl-2 (C-2) from Santa Cruz Biotechnology, Santa Cruz, CA, USA and anti-β-actin from Abcam, Cambridge, UK.