Plastic cassettes

Manufactured by Thermo Fisher Scientific

Sourced in Ireland

Plastic cassettes are laboratory storage and transport containers used to hold and protect samples, reagents, or other materials during various laboratory processes. They are designed to provide a secure and organized environment for the contents while allowing for easy handling and access.

Automatically generated - may contain errors

Lab products found in correlation

Rm2235 rotary microtome, by Leica (2 mentions) Histo clear 2, by National Diagnostics (2 mentions)

2 protocols using plastic cassettes

Brain Tissue Processing for Histology

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Formalin‐fixed brains were placed in plastic cassettes (Thermo Fisher Scientific, Dublin, Ireland) and dehydrated by transfer to 70% ethanol for 20 min followed by 1.5 hourly changes through 70%, 80%, 95%, 100% I, 100% II ethanol before 100% ethanol III overnight and two 5 hr periods and an additional overnight period in Histoclear II (National Diagnostics, Atlanta, GA). Cassettes were then placed through two changes of molten Paraplast paraffin embedding medium (McCormick Scientific, St Louis, MO), heated to 60°C for 2 hr before embedding in molten wax and cooling. Sections (10 μm) were cut on a Leica RM2235 Rotary Microtome (Leica Microsystems, Lab instruments and Supplies, Ashbourne) and floated onto SuperFrost®Plus electrostatically charged slides (Menzel‐Gleser, Braunschweig, Germany) and dried at 37°C overnight.

Nazmi A., Field R.H., Griffin E.W., Haugh O., Hennessy E., Cox D., Reis R., Tortorelli L., Murray C.L., Lopez‐Rodriguez A.B., Jin L., Lavelle E.C., Dunne A, & Cunningham C. (2019). Chronic neurodegeneration induces type I interferon synthesis via STING, shaping microglial phenotype and accelerating disease progression. Glia, 67(7), 1254-1276.

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Brain Tissue Embedding and Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed brains were placed in plastic cassettes (Thermo Fisher Scientific, Dublin, Ireland) and dehydrated by transfer to 70% ethanol for 20 minutes followed by 1.5 hourly changes through 70%, 80%, 95%, 100% I, 100% II ethanol before 100% ethanol III overnight and two five hour periods and an additional overnight period in Histoclear II (National Diagnostics, Atlanta, GA, USA). Cassettes were then placed through two changes of molten Paraplast paraffin embedding medium (McCormick Scientific, St Louis, MO, USA), heated to 60°C for two hours before embedding in molten wax and cooling. Sections (10μm) were cut on a Leica RM2235 Rotary Microtome (Leica Microsystems, Lab instruments and Supplies, Ashbourne) and floated onto SuperFrost®Plus electrostatically charged slides (Menzel-Gleser, Braunschweig, Germany) and dried at 37°C overnight.

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