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Cd34 fluor450

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The CD34-Fluor450 is a fluorescently-labeled antibody that binds to the CD34 antigen, which is expressed on the surface of hematopoietic stem and progenitor cells. It is designed for use in flow cytometry applications to identify and analyze these cell populations.

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Lab products found in correlation

Cd41 fitc, by BioLegend (3 mentions) Cd11b pe cy5, by BioLegend (3 mentions) Human fc receptor binding inhibitor, by Thermo Fisher Scientific (3 mentions) Dxp10 flow cytometer, by Cytek (3 mentions) Cd235 apc, by BioLegend (3 mentions) Cd71 apc, by BD (3 mentions) Cd34 pe cy7, by BioLegend (2 mentions) Gata1 pe, by Cell Signaling Technology (2 mentions) Cd16 32 fitc, by BioLegend (1 mentions) Sca1 pe, by Thermo Fisher Scientific (1 mentions)

3 protocols using cd34 fluor450

1

Cell Surface Flow Cytometry Analysis

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For cell surface flow cytometry, cells were incubated with human Fc receptor binding inhibitor (No. 14-9161-73, eBioscience, San Diego, CA) followed by primary antibodies CD235-APC (No. 306607, BioLegend, San Diego, CA), CD41-FITC (RRID: AB_314373, BioLegend No. 303703), and CD11b-PE/Cy5 (No. 101209, RRID: AB_312995, BioLegend) or CD-71-APC (No. BD551374, RRID: AB_398500, BD Biosciences, Franklin Lakes, NJ), CD34-Fluor450 (No. 48-0341-82, RRID: AB_468936, eBioscience), CD34-PE/Cy7 (No. 343615, RRID: AB_2629725, BioLegend), and GATA1-PE (No. 13353, RRID: AB_2798187, Cell Signaling Technology, Danvers, MA). Data were collected on a DxP10 flow cytometer (Cytek, Cerritos, CA) and analyzed using FlowJo Software, Version 9.7.2.
Wilkes M.C., Scanlon V., Shibuya A., Cepika A.M., Eskin A., Chen Z., Narla A., Glader B., Roncarolo M.G., Nelson S.F, & Sakamoto K.M. (2022). Downregulation of SATB1 by miRNAs reduces megakaryocyte/erythroid progenitor expansion in preclinical models of Diamond–Blackfan anemia. Experimental hematology, 111, 66-78.
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2

Cell Surface Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell surface flow cytometry, cells were incubated with human Fc receptor binding inhibitor (No. 14-9161-73, eBioscience, San Diego, CA) followed by primary antibodies CD235-APC (No. 306607, BioLegend, San Diego, CA), CD41-FITC (RRID: AB_314373, BioLegend No. 303703), and CD11b-PE/Cy5 (No. 101209, RRID: AB_312995, BioLegend) or CD-71-APC (No. BD551374, RRID: AB_398500, BD Biosciences, Franklin Lakes, NJ), CD34-Fluor450 (No. 48-0341-82, RRID: AB_468936, eBioscience), CD34-PE/Cy7 (No. 343615, RRID: AB_2629725, BioLegend), and GATA1-PE (No. 13353, RRID: AB_2798187, Cell Signaling Technology, Danvers, MA). Data were collected on a DxP10 flow cytometer (Cytek, Cerritos, CA) and analyzed using FlowJo Software, Version 9.7.2.
Wilkes M.C., Scanlon V., Shibuya A., Cepika A.M., Eskin A., Chen Z., Narla A., Glader B., Roncarolo M.G., Nelson S.F, & Sakamoto K.M. (2022). Downregulation of SATB1 by miRNAs reduces megakaryocyte/erythroid progenitor expansion in preclinical models of Diamond–Blackfan anemia. Experimental hematology, 111, 66-78.
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3

Cell Surface Marker Analysis by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell surface flow cytometry, cells were incubated with human Fc receptor binding inhibitor (#14-9161−73; eBioscience) followed by primary antibodies CD235−APC (#306607; BioLegend) CD41-FITC (#303703; BioLegend) and CD11b-PE/Cy5 (#101209; BioLegend) or CD-71-APC (BD551374; BD Biosciences), p53-PECy7 (NB200-171; Novus), Sca-1-PE (#12-5981-81; eBioscience), CD34-Fluor®450 (#48-0341-82; eBioscience) and CD16/32-FITC (#101305; BioLegend). FRET measurements were obtained as described69 (link). To measure ECFP and FRET cells were excited with the 405 nm laser and fluorescence was collected in the ECFP channel with a standard 450/40 filter, while the FRET-signal was measured with a 529/24 filter. To measure EYFP, cells were excited with the 488 nm laser while emission was also taken with a 529/24 filter. Data were collected on a DxP10 flow cytometer (Cytek) and analyzed by using FlowJo Software, v.9.7.2.
Wilkes M.C., Siva K., Chen J., Varetti G., Youn M.Y., Chae H., Ek F., Olsson R., Lundbäck T., Dever D.P., Nishimura T., Narla A., Glader B., Nakauchi H., Porteus M.H., Repellin C.E., Gazda H.T., Lin S., Serrano M., Flygare J, & Sakamoto K.M. (2020). Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase. Nature Communications, 11, 3344.
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