TG 18:1_18:1_18:1 was purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-Methoxypropene (MxP) and 2,2′-azobis-(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN) were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Fresh EVOO was collected immediately after production at J-Oil MILLS, Inc. (Tokyo, Japan) and stored under N2 gas and shading until use. All other reagents were of the highest grade available.
2 2 azobis 4 methoxy 2 4 dimethylvaleronitrile meo amvn
Manufactured by Fujifilm
Sourced in Japan
2,2′-azobis-(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN) is a chemical compound used as a radical initiator in various laboratory processes. It functions by generating free radicals through thermal decomposition, which can initiate polymerization or other chemical reactions. The core property of MeO-AMVN is its ability to act as a free radical source under controlled conditions.
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Lab products found in correlation
2 methoxypropene mxp, by Fujifilm (1 mentions)
Cryptoxanthin, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Lycopene, by Merck Group (1 mentions)
Sodium methoxide, by Fujifilm (1 mentions)
Trioctanoyl glycerol, by Merck Group (1 mentions)
2 methoxypropene, by Fujifilm (1 mentions)
Pyridinium p toluenesulfonate, by Merck Group (1 mentions)
Sodium taurocholate, by Nacalai Tesque (1 mentions)
Dioleoyl glycerol, by Fujifilm (1 mentions)
Fatty acid free bovine serum albumin, by Fujifilm (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Isopropyl β d thiogalactoside iptg, by Thermo Fisher Scientific (1 mentions)
Phenylmethylsulfonyl pmsf, by Merck Group (1 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride aaph, by Fujifilm (1 mentions)
Lb broth, by Thermo Fisher Scientific (1 mentions)
4 protocols using 2 2 azobis 4 methoxy 2 4 dimethylvaleronitrile meo amvn
1
Lipid Synthesis Protocol with Specialty Reagents
2
Carotenoid and Antioxidant Quantification
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All-trans-β-carotene (type II), α-carotene, cryptoxanthin, lycopene, α-tocopherol, L-ascorbic acid, β-glucuronidase type II, quercetin, uric acid, human serum albumin, and phosphatidylcholine (type XVI-E) were purchased from Sigma Chemical Co. (St Louis, MO). Lutein was purchased from Kemin Industries (Des Moines, IA). The fatty acid analogue 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undeca noic acid (BODIPY 581/591) was purchased from Molecular Probes (Eugene, OR). The lipophilic radical initiator, 2,2'-azobis (4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN) was purchased from Wako Chemicals (Richmond, VA, USA). Delipidized human serum (DHS) was purchased from SeraCare Life Sciences (Oceanside, CA). All HPLC solvents were obtained from JT Baker Chemical and were filtered through a 0.45-µm membrane filter before use.
3
Enzymatic Synthesis of Lipid Derivatives
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Oleic acid (FA 18:1), trioleoyl glycerol (TG 18:1/18:1/18:1), pyridinium p-toluenesulfonate (PPTS), trioctanoyl glycerol (TG 8:0/8:0/8:0) and deuterated methanol (CH3OD) (99.5 atom % D) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dioleoyl glycerol (DG 18:1/18:1/0:0), 2-methoxypropene (MxP), N,N′-dicyclohexylcarbodiimide (DCC), dimethylaminopyridine (DMAP), 2, 2′-azobis (4-methoxy-2, 4-dimethylvaleronitrile) (MeO-AMVN), fatty acid-free bovine serum albumin and sodium methoxide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Boron trifluoride diethyl ether complex was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Oleic acid methyl ester (FA 18:1; 1OMe) was purchased from Nu-Chek Prep Inc. (Elysian, MN). α-Lipase (neurase F3G) was kindly provided by Amano Enzyme Inc. (Aichi, Japan). Sodium taurocholate was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). All other reagents were of analytical grade or higher.
4
Lipid Oxidation Regulation Assay
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Triton X-100, phenylmethylsulfonyl (PMSF), aprotinin, leupeptin, reduced glutathione, dithiothreitol (DTT), 2, 7-dichlorofluoresceindiacetate (DCFH-DA), hydrogen peroxide (H2O2), low density lipoproteins (LDL), palmitate (PA), alpha-bromo palmitate (α-Br-PA), and DH5α-T1 R competent cells were purchased from Sigma (St. Louis, MO, USA). Site-Directed Mutagenesis system kit, LB broth, and isopropyl-β-D-thiogalactoside (IPTG), were purchased from Fisher Scientific (Ottawa, ON, CA). Mutagenic primers, pGEX 3' Sequencing Primer, 5', GSTrap Fastflow columns, and PreScission Protease were obtained GE Life Sciences, Chicago, USA). Thiobarbituric acid (TBA) was purchased from EMD Millipore (Billerica, MA, USA). 2, 2'-Azobis (2methylpropionamidine) dihydrochloride (AAPH) and 2,2'-Azobis(4-methoxy-2,4dimethylvaleronitrile)
(MeO-AMVN) were purchased from Wako (Osaka, Japan).
(MeO-AMVN) were purchased from Wako (Osaka, Japan).
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