Anti f4 80 monoclonal antibody

Human serum albumin (HSA) was purchased from the Japan Blood Products Organization (Tokyo, Japan). Diphenylene iodonium (DPI), MitoTEMPO, insulin, dexamethasone and isobutylmethylxanthine were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-F4/80 monoclonal antibody was purchased from eBioscience (San Diego, CA, USA). Potassium iodide, chloramine T, acetic acid and N-acetyl-L-cysteine (NAC) were purchased from Nacalai Tesque (Kyoto, Japan). 5-(and 6)-chloromethyl-2′,7′-dicholorodihydrofluorescein diacetate (CM-H2DCFDA) and Dulbecco’s phosphate-buffered saline (D-PBS) were purchased from Invitrogen (Grand Island, NY, USA). Dulbecco’s modified eagle medium (DMEM)-high glucose and DMEM-low glucose were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). All methods were carried out in accordance with approved guidelines. All experimental protocols were approved by Kumamoto University.

IS, 3-indoleacetic acid, kynurenic acid, probenecid, diphenylene iodonium (DPI), insulin, dexamethasone, isobutylmethylxanthine, were purchased from Sigma-Aldrich (St Louis, MO, USA). PCS was synthesized according to a previous report [34 (link)]. The rabbit anti-OAT3 polyclonal antibody (bs-0609R) was purchased from Bioss Inc (Boston, MA, USA). The anti-IS antibody was kindly provided by the Kureha Corporation (Tokyo, Japan). The anti-F4/80 monoclonal antibody was purchased from eBioscience (San Diego, CA, USA). The nitro-Tyr antibody was purchased from Millipore (Burlington, MA, USA). N-acetyl-L-cysteine was purchased from nacalai tesque (Kyoto, Japan). Dulbecco’s phosphate-buffered saline (D-PBS), 5-(and 6)-chloromethyl-2,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) was purchased from Gibco (Invitrogen, Grand Island, NY, USA). Dulbecco’s modified eagle medium (DMEM)-high glucose and DMEM-low glucose was purchased from Wako (Osaka, Japan). Penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). All procedures were carried out in accordance with approved guidelines. All experimental protocols were approved by Kumamoto University.