κB-Luc—luciferase expression driven by NF-κB (cat. number: D2206)—was purchased from Beyotime Biotechnology, China. pRL-TK vector (cat. number: E2231) was from Promega Corporation, which was used as an internal control reporter in combination with any experimental reporter vector to cotransfect MH-S cells. The DEI4-Luc harboring 4 copies of a C/EBP binding site and MCP-1-Luc—luciferase expression driven by MCP-1 promoter—were kindly provided by Dr. Richard C. Schwartz (Michigan State University). Total of 0.5 μg plasmids including 0.45 μg reporter vector and 0.05 μg pRL-TK were introduced into MH-S cells by using Fugene® 6 Transfection Reagent as recommended by the manufacturer. Forty-eight hours later, cells were treated with or without 100 ng/mL of LPS for 4 hours. The cells were then lysed and luciferase expressions were measured by using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).
Prl tk vector
Manufactured by Beyotime
Sourced in China
The PRL-TK vector is a plasmid designed for gene expression studies. It contains the Herpes Simplex Virus Thymidine Kinase (HSV-TK) gene, which can be used as a reporter or selection marker. The vector also includes a multiple cloning site for the insertion of target genes.
Automatically generated - may contain errors
Lab products found in correlation
Dual luciferase reporter assay system, by Promega (1 mentions)
Glial fibrillary acidic protein (gfap), by Proteintech (1 mentions)
Pjet1.2 vector, by Thermo Fisher Scientific (1 mentions)
Tubulin, by Proteintech (1 mentions)
U87mg cell line, by American Type Culture Collection (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg, by Boster Bio (1 mentions)
Aβ 1 16, by BioLegend (1 mentions)
Nc sirna duplex, by GenePharma (1 mentions)
2 protocols using prl tk vector
1
NF-κB and MCP-1 Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
κB-Luc—luciferase expression driven by NF-κB (cat. number: D2206)—was purchased from Beyotime Biotechnology, China. pRL-TK vector (cat. number: E2231) was from Promega Corporation, which was used as an internal control reporter in combination with any experimental reporter vector to cotransfect MH-S cells. The DEI4-Luc harboring 4 copies of a C/EBP binding site and MCP-1-Luc—luciferase expression driven by MCP-1 promoter—were kindly provided by Dr. Richard C. Schwartz (Michigan State University). Total of 0.5 μg plasmids including 0.45 μg reporter vector and 0.05 μg pRL-TK were introduced into MH-S cells by using Fugene® 6 Transfection Reagent as recommended by the manufacturer. Forty-eight hours later, cells were treated with or without 100 ng/mL of LPS for 4 hours. The cells were then lysed and luciferase expressions were measured by using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).
+ Expand
κB-Luc—luciferase expression driven by NF-κB (cat. number: D2206)—was purchased from Beyotime Biotechnology, China. pRL-TK vector (cat. number: E2231) was from Promega Corporation, which was used as an internal control reporter in combination with any experimental reporter vector to cotransfect MH-S cells. The DEI4-Luc harboring 4 copies of a C/EBP binding site and MCP-1-Luc—luciferase expression driven by MCP-1 promoter—were kindly provided by Dr. Richard C. Schwartz (Michigan State University). Total of 0.5 μg plasmids including 0.45 μg reporter vector and 0.05 μg pRL-TK were introduced into MH-S cells by using Fugene® 6 Transfection Reagent as recommended by the manufacturer. Forty-eight hours later, cells were treated with or without 100 ng/mL of LPS for 4 hours. The cells were then lysed and luciferase expressions were measured by using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).
2
Dicer1 Knockdown Modulates Alzheimer's Pathology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following primary antibodies were used in this study:Dicer1(Sigma,St Louis, MO,USA,cat# WH0023405M1,RRID:AB_1841286),GFAP(Proteintech,Wuhan,China, cat#16825-1-AP,RRID:2109646),ApoE(Thermo Fisher Scientific,Carlsbad,CA,USA, Cat#701241,RRID:AB_2532438),Tubulin(Proteintech,Cat#14555-1-AP, RRID:AB_2212258),Aβ(1-16)(BioLegend,San Diego,CA,USA,Cat# 803001,RRID: AB_2721329). The following secondary antibodies were also used in this study:goat anti-mouse horseradish peroxidase conjugated IgG (Boster Biological Technology,Co.
Ltd,Wuhan,China),goat anti-Rabbit horseradish peroxidase conjugated IgG(Boster Biological Technology). The following reagents or cell lines were used in this study:the Dicer1 siRNA duplex and the negative control(NC) siRNA duplex (Genepharma, Suzhou,China),pfu High fidelity enzyme(Qiagen,Beijing,China,cat#KP202),pJet1.2 vector(Thermo Fisher Scientific,Carlsbad,CA,USA,cat# K1231),pGL6-miR-basic (Beyotime Biotechnology,Haimen,China),pRL-TK Vector(Beyotime Biotechnology,Inc, cat#D2762),a human pCMV-Dicer 1(Sino Biological,Beijing,China,cat# HG11350-NH), U87MG cell line(ATCC,Manassas,VA cat#HTB-14,RRID:CVCL_0022).
Ltd,Wuhan,China),goat anti-Rabbit horseradish peroxidase conjugated IgG(Boster Biological Technology). The following reagents or cell lines were used in this study:the Dicer1 siRNA duplex and the negative control(NC) siRNA duplex (Genepharma, Suzhou,China),pfu High fidelity enzyme(Qiagen,Beijing,China,cat#KP202),pJet1.2 vector(Thermo Fisher Scientific,Carlsbad,CA,USA,cat# K1231),pGL6-miR-basic (Beyotime Biotechnology,Haimen,China),pRL-TK Vector(Beyotime Biotechnology,Inc, cat#D2762),a human pCMV-Dicer 1(Sino Biological,Beijing,China,cat# HG11350-NH), U87MG cell line(ATCC,Manassas,VA cat#HTB-14,RRID:CVCL_0022).
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