BECs grown in EGM2 complete medium for 1 day were used for this experiment. After washing with HBSS, cells were treated either with vehicle (DMSO) or 17β-Oestradiol (10μM) for 16 hrs in Glucose-, Glutamine- and serum-free endothelial cell medium (cell biologics) and supplemented either with 5mM Glucose, 2mM Glutamine or 20mM Linoleic acid-Oleic acid (Sigma) to determine the effect of nutrient supplementation on cell growth. Cells were stained with anti-Ki-67 (Abcam, 1:300) to measure EC proliferation or with cleaved caspase-3 (Cell Signalling Technology, 1:300) to measure apoptosis.
Linoleic acid oleic acid
Manufactured by Merck Group
Linoleic acid-Oleic acid is a chemical compound consisting of two fatty acids commonly found in various natural sources. It serves as a fundamental component in the formulation and production of various laboratory reagents and materials. The core function of this compound is to provide a stable and standardized source of these essential fatty acids for use in diverse analytical and experimental procedures.
Automatically generated - may contain errors
2 protocols using linoleic acid oleic acid
1
Nutrient Effects on Endothelial Cell Growth
2
Nutrient Effects on Endothelial Cell Growth
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