Log In Sign up
The largest database of trusted experimental protocols

Mouse anti histone 3

Manufactured by Abcam
Visit Supplier

Mouse anti-Histone 3 is a primary antibody that specifically recognizes the histone H3 protein. Histones are core components of chromatin and play a crucial role in the regulation of gene expression. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP).

Automatically generated - may contain errors

Lab products found in correlation

Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Sheep anti mouse hrp, by GE Healthcare (2 mentions) Sds page 10 bis tris gel, by Thermo Fisher Scientific (2 mentions) Rabbit anti tfap2c, by Abcam (2 mentions) Donkey anti goat hrp, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Deltavision omx sr microscope, by GE Healthcare (1 mentions)

Close spelling variants of this product

Histone 3 (45 citations) Anti-Histone 3 (19 citations) Mouse anti (2 citations)

3 protocols using mouse anti histone 3

1

Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were briefly washed in PBS and then fixed in PBS containing 4% paraformaldehyde (Sigma) for 10 min at RT. Fixed cells were washed in PBS and were then permeabilized in PBS with 0.2% Triton X‐100 for 5 min at RT. Cells were blocked in PBS with 4% BSA (Sigma). Primary and secondary antibody incubations were performed in PBS with 1% BSA overnight at 4°C and 1 h at RT, respectively. Slides were imaged on a Leica TCS sp8 STED or GE DeltaVision OMX SR microscope. Antibodies used include mouse anti‐HA (CST), rabbit anti‐hTrmt13, and mouse anti‐Histone 3 (Abcam).
Li H., Dong H., Xu B., Xiong Q., Li C., Yang W., Li J., Huang Z., Zeng Q., Wang E, & Liu R. (2021). A dual role of human tRNA methyltransferase hTrmt13 in regulating translation and transcription. The EMBO Journal, 41(6), e108544.
+ Open protocol
+ Expand
2

Quantifying TFAP2C and Histone 3 in hESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysate of hESCs was prepared in RIPA buffer and NuPAGE® LDS sample loading buffer and denatured for 10 min at 98°C. Samples were run on an SDS- PAGE 10% Bis-Tris gel (ThermoFisher) 60–80 min at 100V, transferred at 100V for 60–70 min, and blocked with 5% non-fat dried milk in 0.1%TBST (0.1% Tween in Tris Buffer Saline solution) for 1 hr. Primary antibody was added to blocking buffer and incubated at room temperature for 1 hr. Secondary antibodies were added after washing with TBST. The PierceTM ECL Western Blotting Substrate (ThermoScientific, 32109) was used on the membrane before film exposure. The primary antibodies used in this study include: rabbit-anti-TFAP2C (Abcam, 76007, 1:1000), mouse-anti-Histone 3 (Abcam, 10799, 1:8000). The secondary antibodies used include: Donkey-anti-goat HRP (Invitrogen, A15999, 1:2000), Sheep-anti-mouse HRP (GE Healthcare Life Sciences, NA931VS, 1:2000).
Chen D., Liu W., Zimmerman J., Pastor W.A., Kim R., Hosohama L., Ho J., Aslanyan M., Gell J.J., Jacobsen S.E, & Clark A.T. (2018). The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell reports, 25(13), 3591-3602.e5.
+ Open protocol
+ Expand
3

Quantifying TFAP2C and Histone 3 in hESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysate of hESCs was prepared in RIPA buffer and NuPAGE® LDS sample loading buffer and denatured for 10 min at 98°C. Samples were run on an SDS- PAGE 10% Bis-Tris gel (ThermoFisher) 60–80 min at 100V, transferred at 100V for 60–70 min, and blocked with 5% non-fat dried milk in 0.1%TBST (0.1% Tween in Tris Buffer Saline solution) for 1 hr. Primary antibody was added to blocking buffer and incubated at room temperature for 1 hr. Secondary antibodies were added after washing with TBST. The PierceTM ECL Western Blotting Substrate (ThermoScientific, 32109) was used on the membrane before film exposure. The primary antibodies used in this study include: rabbit-anti-TFAP2C (Abcam, 76007, 1:1000), mouse-anti-Histone 3 (Abcam, 10799, 1:8000). The secondary antibodies used include: Donkey-anti-goat HRP (Invitrogen, A15999, 1:2000), Sheep-anti-mouse HRP (GE Healthcare Life Sciences, NA931VS, 1:2000).
Chen D., Liu W., Zimmerman J., Pastor W.A., Kim R., Hosohama L., Ho J., Aslanyan M., Gell J.J., Jacobsen S.E, & Clark A.T. (2018). The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell reports, 25(13), 3591-3602.e5.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!