Aliquots (2 µL) of web extracts of adult and subadult female S. triangulosa were analyzed, and compared, using coupled high-performance liquid chromatography—mass spectrometry (HPLC/MS). The Bruker maXis Impact Quadrupole Time-of-Flight LC/MS system consisted of an Agilent 1200 LC fitted with a Spursil C18 column (30 mm × 3.0 mm, 3 µm; Dikma Technologies, Foothill Ranch, CA, USA) and a Bruker maXis Impact Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer. The LC/MS was operated with positive electrospray ionisation (+ ESI) at a gas temperature of 200 °C and a flow of 9 L/min. The nebuliser was set to 4 bar and the capillary voltage to 4200 V. The column was eluted with a 0.4 mL/min flow of a solvent gradient, starting with 80% water and 20% acetonitrile, and ending with 100% acetonitrile after 4 min. The solvent system contained 0.1% formic acid to improve the peak shape of compounds.
Ultra high resolution tandem tof uhr qq tof mass spectrometer
Manufactured by Bruker
Sourced in United States
The Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer is a laboratory instrument designed for high-performance mass analysis. It utilizes a combination of time-of-flight (TOF) and quadrupole (Qq) technologies to provide ultra-high mass resolution and sensitivity for a wide range of applications.
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2 protocols using ultra high resolution tandem tof uhr qq tof mass spectrometer
1
HPLC-MS Analysis of Spittlebug Extracts
2
HPLC Analysis of Rhizoferrin Identification
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High pressure liquid chromatography (HPLC) was used to verify the presence of rhizoferrin. Rhizoferrin was purified from cultures as described above and dried or freeze-dried samples were re-constituted in water. Ten microliters were injected into a Gemini C18 column (Phenomenex, 250 x 4.6 mm, 5 µm particle size) and compounds were separated using a gradient of 2% -40% solvent A over 25 minutes using a flow rate of 1 ml/min. Solvent A consisted of acetonitrile (Fisher Scientific, HPLC grade) plus 0.2% trifluoroacetic acid (Caledon Laboratories). Solvent B was water. Absorbance was monitored at 220 nm. To confirm the presence of rhizoferrin, fractions were collected and mass spectrometry was performed using the Agilent 1200 HPLC and a Bruker maXis Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer. Samples were introduced by flow injection via HPLC with acetonitrile/water (0.1 % formic acid) as mobile phase and spectra were collected under positive electrospray ionization (+ESI).
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