THP‐1 cells were treated with 10 μM of designated drug or vehicle (DMSO), and 24 h after treatment supernatant was added to Human Cytokine XL proteome array (R&D Systems) in accordance with manufacturer protocol. Chemiluminescence was used to visualize protein quantities. Cytokine analysis from human PBMC's was done on MSD plates as described by manufacturer protocol.
Human cytokine xl proteome array
Manufactured by R&D Systems
The Human Cytokine XL Proteome Array is a multiplex assay that allows simultaneous detection and quantification of 105 different human cytokines, chemokines, and other soluble proteins in a single experiment. The array utilizes an optimized panel of carefully selected capture antibodies printed on nitrocellulose-coated glass slides. This technology enables researchers to obtain a comprehensive profile of the complex cytokine network from a small sample volume.
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Lab products found in correlation
4 protocols using human cytokine xl proteome array
1
Cytokine Profiling in THP-1 Cells
2
Cytokine and Angiogenesis Profiling
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THP-1 cells were differentiated as previously described in this manuscript. Following differentiation, cells were treated with 10 μM Takinib or DMSO vehicle control. 24 hours after treatment, supernatant was added to Human Cytokine XL proteome array (R&D Systems), or Angiogenesis proteome array. Apoptosis biomarkers were visualized with the Apoptosis Array kit (R&D Systems). All procedures were conducted in accordance with manufacturer protocol. Chemiluminescence was used to visualize protein quantities.
3
Cytokine Profiling in THP-1 Cells
4
THP-1 Cytokine Profiling with Takinib
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THP-1 Cells were differentiated as previously described in this manuscript. Following differentiation, cells were treated with 10 μM Takinib or DMSO. 24 hours after treatment, supernatant was added to Human Cytokine XL proteome array (R&D Systems). Cytokine kit was conducted in accordance with manufacturer protocol. Chemiluminescence was used to visualize protein quantities.
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