Over the counter compete dry dog foods (41) for adult dogs were selected in packages from the range of 0.3 to 2.5 kg. These foods were randomly chosen at Polish pet stores and online stores. Dog foods were bought in January 2022. Foods were divided into groups depending on the main animal component: fish (F), poultry (P), lamb (L), beef (B). A group of mixed foods (M) was distinguished, which contained at least two different sources of animal components, and a group of other foods (O) with unconventional sources of animal protein (Table S1 ). All bags were stored in laboratory room temperature (approx. 18–21 °C) until analyzes. Bags were opened on the same day. From each bag, a representative sample was collected for laboratory analyzes52 . Samples of foods were ground in a laboratory mill type KNIFETEC 1095 (Foss Tecator, Höganäs, Sweden), placed in sterile containers and marked with the symbols 1–41 (Tables 1 , 2 , 3 , 4 , 5 ).
Knifetec 1095
Manufactured by Foss
Sourced in Denmark
The Knifetec™ 1095 is a laboratory equipment designed for precision cutting tasks. It features a high-quality stainless steel blade and a sturdy, ergonomic handle. The device is intended for use in controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Rutin, by ChromaDex (1 mentions)
Kaempferol, by ChromaDex (1 mentions)
Myricetin, by ChromaDex (1 mentions)
Quercetin, by ChromaDex (1 mentions)
Mini protean tetra cell, by Bio-Rad (1 mentions)
Image lab 4, by Bio-Rad (1 mentions)
Amersham low molecular weight calibration kit for sds electrophoresis, by GE Healthcare (1 mentions)
Coomassie brilliant blue r 250 staining solution, by Bio-Rad (1 mentions)
4 protocols using knifetec 1095
1
Nutritional Analysis of Commercially Available Dry Dog Foods
2
Chamomile Phytochemical Characterization
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Nineteen samples of chamomile were obtained from different manufacturers in Poland and are listed in Table 1 with their characterizations. The samples were homogenized at 20°C in a water-cooled grinder Knifetec 1095 (Foss Tecator, Höganäs, Sweden) and stored in desiccator which was protected from light.
Standards of phenolic acids—gallic, caffeic, syringic, p-coumaric, ferulic, and of flavonoids—rutin, myricetin, quercetin and kaempferol were purchased from ChromaDex (California, USA). HPLC grade methanol and acetonitrile were purchased from POCh (Gliwice, Poland) and J.T. Baker (Phillipsbusg, USA), respectively. Analytical grade ethanol, methanol, acetic acid, ferric chloride hexahydrate, 6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid (Trolox), 2,4,6-tris (2-pyridyl)-1,3,5-triazine (TPTZ), and 1,1-diphenyl-2-picrylhydrazol radical (DPPH) were purchased from POCh (Gliwice, Poland). Trifluoroacetic acid (TFA) was obtained from Sigma Aldrich (St. Louis, MO, USA). Redistillated water was prepared by triple distillation of water in a Destamat Bi-18 system (Heraeus Quarzglas, Hanau, Germany).
Standards of phenolic acids—gallic, caffeic, syringic, p-coumaric, ferulic, and of flavonoids—rutin, myricetin, quercetin and kaempferol were purchased from ChromaDex (California, USA). HPLC grade methanol and acetonitrile were purchased from POCh (Gliwice, Poland) and J.T. Baker (Phillipsbusg, USA), respectively. Analytical grade ethanol, methanol, acetic acid, ferric chloride hexahydrate, 6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid (Trolox), 2,4,6-tris (2-pyridyl)-1,3,5-triazine (TPTZ), and 1,1-diphenyl-2-picrylhydrazol radical (DPPH) were purchased from POCh (Gliwice, Poland). Trifluoroacetic acid (TFA) was obtained from Sigma Aldrich (St. Louis, MO, USA). Redistillated water was prepared by triple distillation of water in a Destamat Bi-18 system (Heraeus Quarzglas, Hanau, Germany).
3
Characterization of Japonica Rice Cultivars
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Seeds of O. sativa L. spp. japonica, cvs Arborio, Volano, Carnaroli, and Karnak, were kindly provided by the Rice Research Unit, CREA (Vercelli, Italy). The main characteristics of each cvs are reported in Table S1 . Commercial samples of Carnaroli and Karnak were purchased from local market. For both proteomics and genomics analyses, seeds were milled with the refrigerated sample mill Knifetec™ 1095 (Foss) to obtain a fine powder.
4
Quantification of Wheat Gluten Proteins
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Grain samples of 35 g from each cv. were milled to a fine powder with a Knifetec™ 1095 (Foss, Hillerød, Denmark). Gluten proteins (gliadins, HMW-GS, and LMW-GS) were extracted from 30-mg samples and quantified following a previous reported methodology [23] (link).
Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed in a Mini-PROTEAN Tetra Cell (Bio-Rad) using 8% acrylamide gels for HMW-GS, and 12% acrylamide gels for gliadins and LMW-GS. Aliquots of 20 µg of each dried protein were suspended in 20 µL of loading buffer containing 20 g L -1 SDS, 0.2 g L -1 bromophenol blue, 1 mL L -1 β-mercaptoethanol, 0.05 mol L -1 Tris HCl (pH 6.8), and 100 mL L -1 glycerol, then boiled at 95 • C for 5 min before loading onto the gels. An Amersham Low Molecular Weight Calibration Kit for SDS Electrophoresis (MW 14,400-97,000 Da) (GE Healthcare, Chicago, IL, USA) was used to detect HMW, LMW-GS and gliadin bands. After electrophoretic separation at 40 mA, the gels were fixed in 70 mL L -1 acetic acid and 400 mL L -1 methanol and stained with Coomassie Brilliant Blue R-250 Staining Solution (Bio-Rad). Image Lab 4.5.1 software (Bio-Rad) was used for relative quantification of the glutenin subunits on each gel. Gliadins were divided into three classes (ω, α/β and γ) based on their molecular weight.
Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed in a Mini-PROTEAN Tetra Cell (Bio-Rad) using 8% acrylamide gels for HMW-GS, and 12% acrylamide gels for gliadins and LMW-GS. Aliquots of 20 µg of each dried protein were suspended in 20 µL of loading buffer containing 20 g L -1 SDS, 0.2 g L -1 bromophenol blue, 1 mL L -1 β-mercaptoethanol, 0.05 mol L -1 Tris HCl (pH 6.8), and 100 mL L -1 glycerol, then boiled at 95 • C for 5 min before loading onto the gels. An Amersham Low Molecular Weight Calibration Kit for SDS Electrophoresis (MW 14,400-97,000 Da) (GE Healthcare, Chicago, IL, USA) was used to detect HMW, LMW-GS and gliadin bands. After electrophoretic separation at 40 mA, the gels were fixed in 70 mL L -1 acetic acid and 400 mL L -1 methanol and stained with Coomassie Brilliant Blue R-250 Staining Solution (Bio-Rad). Image Lab 4.5.1 software (Bio-Rad) was used for relative quantification of the glutenin subunits on each gel. Gliadins were divided into three classes (ω, α/β and γ) based on their molecular weight.
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