4-(Methylnitrosoamino)-1-(3-pyridinyl)-1-butanone (NNK, Cat No. 78013), N′-Nitrosonornicotine (NNN, Cat No. 75285), 4-Nitroquinoline N-oxide (4-NQO, Cat No. N8141), Cisplatin (PHR1624), and 5-Flurouracil (5-FU) (F6627) were purchased from Sigma-Aldrich, Saint Louis, MO, USA.
Cisplatin phr1624
Manufactured by Merck Group
Sourced in United States
Cisplatin (PHR1624) is a chemical compound used as a laboratory reagent. It is a platinum-based drug that can be used in various research applications. The core function of Cisplatin is to act as a chemotherapeutic agent, which can be utilized in the study of cancer cell biology and drug development processes.
Automatically generated - may contain errors
Lab products found in correlation
4 nitroquinoline n oxide 4 nqo, by Merck Group (1 mentions)
Ki67 ab16667, by Abcam (1 mentions)
Vimentin 60330 1 ig, by Proteintech (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Tunel kit, by Beyotime (1 mentions)
Bay 11 7 082 hy 13453, by MedChemExpress (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Eca109, by National Collection of Authenticated Cell Cultures (1 mentions)
Het 1a, by National Collection of Authenticated Cell Cultures (1 mentions)
3 protocols using cisplatin phr1624
1
Procuring Carcinogenic Compounds for Research
2
Cisplatin-Induced Apoptosis in IRF6-Overexpressing Cells
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Cisplatin (PHR1624) was supplied by Sigma. IRF6 overexpression (IRF6-OE) and negative control (NC) were supplied by Shanghai Yuanke Biotechnology Co., Ltd. CCK8 cell proliferation and cytotoxicity assay kit (HY-K0301) were purchased from MCE. TUNEL kit (C1091) was purchased from Beyotime. lipofectamine 2000 (11668–019) was purchased from Invitrogen. Primary antibodies of Caspase-3 (19677-1-AP) and Vimentin (60330-1-Ig) were purchased from Proteintech. Antibodies of IRF6 (PA5-84583), BAX (MA5-32031) and Bcl2 (MA1-12246) were purchased from Invitrogen and E-cadherin (ab231303) as well as Ki67 (ab16667) from Abcam.
3
Esophageal Cancer Cell Line Maintenance
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Human esophageal epithelial cell line Het-1A and human ESCC cell lines TE-1 and Eca109 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All the cell lines were maintained in RPMI medium (Gibco), supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100-mg/ml streptomycin and incubated at 37 °C in a humidified atmosphere containing 5% CO2.
TAPI-1 (HY-16657) and BAY11-7082 (HY-13453) were purchased from MedChem Express and dissolved in dimethyl sulfoxide (DMSO) for storage at – 80 °C. Cisplatin (PHR1624) was obtained from Sigma-Aldrich and dissolved in phosphate-buffered saline (PBS) for storage at – 20 °C.
TAPI-1 (HY-16657) and BAY11-7082 (HY-13453) were purchased from MedChem Express and dissolved in dimethyl sulfoxide (DMSO) for storage at – 80 °C. Cisplatin (PHR1624) was obtained from Sigma-Aldrich and dissolved in phosphate-buffered saline (PBS) for storage at – 20 °C.
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