Thermal cycler dice real time system tp800 2.01c

Manufactured by Takara Bio

The Thermal Cycler Dice Real-Time System TP800 2.01C is a laboratory instrument used for DNA amplification. It is capable of performing real-time PCR (polymerase chain reaction) experiments.

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Lab products found in correlation

Sybr premix ex taq 2, by Takara Bio (1 mentions) Sybr green pcr mix, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thermal Cycler Dice Real Time System II TP800 Thermal Cycler Dice Real Time System TP800 Thermal Cycler Dice Real Time System II Thermal Cycler Dice Real Time System Thermal Cycler Dice TP800 system Dice Real Time System TP800 Thermal Cycler Dice Real Time Thermal Cycler Dice TP800 Cycler Dice Real-Time system Thermal Cycler Dice System 2

2 protocols using thermal cycler dice real time system tp800 2.01c

Quantitative RT-PCR Gene Expression Analysis

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Quantitative real-time RT-PCR was performed with a Thermal Cycler Dice Real-Time System TP800 2.01C (Takara Bio). cDNA (corresponding to 10 ng of total RNA) was subjected to real-time PCR amplification analysis using SYBR Premix Ex Taq II (Takara Bio). The relative quantification method was used to measure the amounts of the respective genes normalized to ACTB. All primers used are shown in Supplementary Table 7.

Tamura R., Yoshihara K., Saito T., Ishimura R., Martínez-Ledesma J.E., Xin H., Ishiguro T., Mori Y., Yamawaki K., Suda K., Sato S., Itamochi H., Motoyama T., Aoki Y., Okuda S., Casingal C.R., Nakaoka H., Inoue I., Verhaak R.G., Komatsu M, & Enomoto T. (2018). Novel therapeutic strategy for cervical cancer harboring FGFR3-TACC3 fusions. Oncogenesis, 7(1), 4.

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Quantitative RT-PCR for Gene Expression

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Quantitative real-time RT-PCR was performed with a Thermal Cycler Dice Real-Time System TP800 2.01C (Takara Bio). cDNA (corresponding to 5 ng of total RNA) was subjected to real-time PCR amplification analysis using SYBR Green PCR mix (Applied Biosystems). Gene expression was tested in a set of 15 human housekeeping genes (Takara Bio) and HPRT1 was selected as having the lowest variation in gene expression. The relative quantification method44 (link) was used to measure the amounts of the respective genes normalized to HPRT1. All primers used in quantitative real-time RT-PCR are shown in Supplementary Table S7.

Tamura R., Yoshihara K., Yamawaki K., Suda K., Ishiguro T., Adachi S., Okuda S., Inoue I., Verhaak R.G, & Enomoto T. (2015). Novel kinase fusion transcripts found in endometrial cancer. Scientific Reports, 5, 18657.

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