Alexa fluor 680 donkey anti mouse igg h l

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 680 donkey anti-mouse IgG (H + L) is a secondary antibody conjugated with the Alexa Fluor 680 fluorescent dye. It is designed to detect and visualize mouse primary antibodies in various immunoassays and imaging applications.

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Alexa Fluor 680 anti-mouse IgG Donkey anti-mouse IgG (H + L) Alexa Fluor IgG H + L Anti-mouse alexa fluor 680 Alexa Fluor donkey anti-mouse Alexa Fluor H + L Alexa Fluor 680 Anti-mouse Alexa Fluor H + L IgG Alexa 680

3 protocols using alexa fluor 680 donkey anti mouse igg h l

Western Blot Analysis of Protein Expression

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Protein lysates were separated using SDS-PAGE and transferred to nitrocellulose membranes (Amersham Pharmacia Biotech, Piscataway, NJ, United States). Membranes were probed with the appropriate primary antibodies (Supplementary Table 2). Alexa Fluor 680 donkey anti-mouse IgG (H + L) or Alexa Fluor 680 donkey anti-rabbit IgG (H + L) were used as secondary antibodies (1:5000; Invitrogen). Fluorophores were detected using the Odyssey Infrared Imaging System (Li-Cor, Lincoln, NE, United States).

Sun C., Wang F.J., Zhang H.G., Xu X.Z., Jia R.C., Yao L, & Qiao P.F. (2017). miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-β/Smad4 pathway. World Journal of Gastroenterology, 23(10), 1816-1827.

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Western Blot Analysis of EMT Markers

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Protein lysates were separated using 8 % or 10 % SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (Amersham Pharmacia Biotech, USA). The membrane was probed with the following antibodies: anti-Smad4, anti-Snail, anti-E-cadherin, anti-N-cadherin (Santa Cruz Biotechnology, USA). Finally, the membrane was probed with Alexa Fluor® 680 donkey anti-mouse IgG (H + L) (1:5000) (Invitrogen, USA). Antibody binding was detected by Odyssey™ Infrared Imaging System (Li-Cor, Lincoln, NE). The names of the antibodies are listed in Additional file 2: Table S2.

Qiao P., Li G., Bi W., Yang L., Yao L, & Wu D. (2015). microRNA-34a inhibits epithelial mesenchymal transition in human cholangiocarcinoma by targeting Smad4 through transforming growth factor-beta/Smad pathway. BMC Cancer, 15, 469.

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Protein Expression Analysis by Western Blot

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Protein lysates were separated using desired SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (Amersham Pharmacia Biotech, USA). The membrane was probed with the following antibodies: anti-PTEN, anti-β-catenin, anti-c-Jun, anti-MMP2, anti-E-cadherin and anti-N-cadherin (Santa Cruz Biotechnology, USA). Finally, the membrane was probed with Alexa Fluor^® 680 donkey anti-mouse IgG (H+L) (Invitrogen). Antibody binding was detected by Odyssey^™ Infrared Imaging System (Li-Cor, Lincoln, NE). The names of the antibodies are listed in S2 Table.

Li J., Yao L., Li G., Ma D., Sun C., Gao S., Zhang P, & Gao F. (2015). miR-221 Promotes Epithelial-Mesenchymal Transition through Targeting PTEN and Forms a Positive Feedback Loop with β-catenin/c-Jun Signaling Pathway in Extra-Hepatic Cholangiocarcinoma. PLoS ONE, 10(10), e0141168.

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