Blood was collected as described above in K2 EDTA tubes at the end of 3-week HDE repetitive dust exposure, and plasma was isolated following centrifugation at 1500 g for 10 min. Samples were kept at −80°C until analysis. Lipids were extracted from plasma as previously described [33 (link)]. Separation of lipids was performed using a UPLC BEH C18 column (2.1 × 50 mm i.d.; 1.7 μm, Waters Corporation, Milford, MA, USA) with an Acquity guard column (UPLC BEH C18 VanGuard Pre-column; 2.1 × 5 mm, i.d., 1.7 μm, Waters Corporation) and detection was performed using UPLC-MS/MS (ultra-performance liquid chromatography tandem mass spectrometry) [33 (link)]. Data acquisition was performed using Acquity I Class UPLC/Xevo TQ-S Micro Mass Spectrometer (Waters Corporation). UPLC and MS parameters for the detection of arachidonoylethanolamide (AEA), 2-arachidonoyl-sn-glycerol (2-AG), 2-docosahexaenoyl-sn-glycerol (2-DG), docosahexaenoylethanolamide (DHEA) and oleoylethanolamide (OEA) were reported previously [34 (link),35 (link)].
Acquity 1 class uplc xevo tq s micro mass spectrometer
Manufactured by Waters Corporation
The Acquity I Class UPLC/Xevo TQ-S Micro Mass Spectrometer is a high-performance liquid chromatography-mass spectrometry (LC-MS) system designed for sensitive and accurate analysis of small molecules. It combines the Acquity I Class UPLC system for efficient separation with the Xevo TQ-S Micro triple quadrupole mass spectrometer for precise quantification and identification of target analytes.
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2 protocols using acquity 1 class uplc xevo tq s micro mass spectrometer
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Lipid Profiling in Dust Exposure
2
Lipid Profiling in Dust Exposure
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Blood was collected as described above in K2 EDTA tubes at the end of 3-week HDE repetitive dust exposure, and plasma was isolated following centrifugation at 1500 g for 10 min. Samples were kept at −80°C until analysis. Lipids were extracted from plasma as previously described [33 (link)]. Separation of lipids was performed using a UPLC BEH C18 column (2.1 × 50 mm i.d.; 1.7 μm, Waters Corporation, Milford, MA, USA) with an Acquity guard column (UPLC BEH C18 VanGuard Pre-column; 2.1 × 5 mm, i.d., 1.7 μm, Waters Corporation) and detection was performed using UPLC-MS/MS (ultra-performance liquid chromatography tandem mass spectrometry) [33 (link)]. Data acquisition was performed using Acquity I Class UPLC/Xevo TQ-S Micro Mass Spectrometer (Waters Corporation). UPLC and MS parameters for the detection of arachidonoylethanolamide (AEA), 2-arachidonoyl-sn-glycerol (2-AG), 2-docosahexaenoyl-sn-glycerol (2-DG), docosahexaenoylethanolamide (DHEA) and oleoylethanolamide (OEA) were reported previously [34 (link),35 (link)].
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