We used the Th1/Th2 U-PLEX (K15069L-2; Meso Scale Discovery [MSD]) platform to analyze cytokines in protein extract and BAL supernatant. This platform uses a 10-spot U-PLEX plate and unique linkers to analyze samples. Biotinylated capture Abs are assigned to linkers, which correspond to unique spots in the U-PLEX plate. The analytes tested were IFN-γ, IL-1β, IL-2, IL-4, IL-5, KC, IL-10, IL-12p70, TNF-α, and IL-13. The MSD instrument applies a voltage to the plate electrodes, causing the capture labels to emit light. The intensity of the emitted light is measured. The BAL supernatant was at 1:1 dilution, and the protein extraction was at 1:2 dilution. Dilutions were used in the assay and then corrected for in the analysis. Blood was collected via cardiac puncture and stored in K2EDTA tubes. The tubeswere centrifuged at 500 × g for 10 min, and the plasma was collected. We used the Mouse IgE ELISA Kit (EMIGHE; Thermo Fisher Scientific) to analyze for IgE. The plasma was diluted 10,000-fold as suggested by the vendor and corrected for in the analysis. The lung protein was extracted from homogenized lung tissue. We used the IL-17 ELISA Kit (GR3332177–1; Abcam) to analyze for IL-17 cytokine. The lung protein extract was diluted 1:1. The dilution was corrected for in the analysis.
Il 17 elisa kit
Manufactured by Abcam
The IL-17 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of interleukin-17 (IL-17) levels in biological samples.
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Lab products found in correlation
2 protocols using il 17 elisa kit
1
Comprehensive Cytokine Profiling in Murine Models
2
Comprehensive Cytokine Profiling in Murine Models
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We used the Th1/Th2 U-PLEX (K15069L-2; Meso Scale Discovery [MSD]) platform to analyze cytokines in protein extract and BAL supernatant. This platform uses a 10-spot U-PLEX plate and unique linkers to analyze samples. Biotinylated capture Abs are assigned to linkers, which correspond to unique spots in the U-PLEX plate. The analytes tested were IFN-γ, IL-1β, IL-2, IL-4, IL-5, KC, IL-10, IL-12p70, TNF-α, and IL-13. The MSD instrument applies a voltage to the plate electrodes, causing the capture labels to emit light. The intensity of the emitted light is measured. The BAL supernatant was at 1:1 dilution, and the protein extraction was at 1:2 dilution. Dilutions were used in the assay and then corrected for in the analysis. Blood was collected via cardiac puncture and stored in K2EDTA tubes. The tubeswere centrifuged at 500 × g for 10 min, and the plasma was collected. We used the Mouse IgE ELISA Kit (EMIGHE; Thermo Fisher Scientific) to analyze for IgE. The plasma was diluted 10,000-fold as suggested by the vendor and corrected for in the analysis. The lung protein was extracted from homogenized lung tissue. We used the IL-17 ELISA Kit (GR3332177–1; Abcam) to analyze for IL-17 cytokine. The lung protein extract was diluted 1:1. The dilution was corrected for in the analysis.
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