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Oti1e4

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OTI1E4 is a lab equipment product made by OriGene. It is a device used for genetic analysis and research purposes.

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Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (6 mentions) Mitotracker red, by Thermo Fisher Scientific (3 mentions) Nhs fitc, by Thermo Fisher Scientific (2 mentions) Anti penta his, by Qiagen (2 mentions) Wiskostatin, by Merck Group (1 mentions) Anti β actin ma5 15739, by Thermo Fisher Scientific (1 mentions) Nhs rhodamine, by Thermo Fisher Scientific (1 mentions) Alkaline phosphatase, by Promega (1 mentions) Cellmask orange, by Thermo Fisher Scientific (1 mentions) Anti gst, by Cell Signaling Technology (1 mentions) Ma5 15256, by Thermo Fisher Scientific (1 mentions) Texas red dye, by Thermo Fisher Scientific (1 mentions) Nocodazole, by Merck Group (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Sir tubulin, by Spirochrome (1 mentions)

2 protocols using oti1e4

1

Antibody Sources and Reagents for Cellular Studies

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The primary antibody against SNX9 (OTI1E4, 1:1000) was purchased from Origene, anti-Pacsin (PA5-83983, 1:200), anti-N-WASP (PA5-52198, 1:200) and anti-β-actin (MA5-15739, 1:2000) antibodies were sourced from Thermo Scientific and the anti-actin (#7301-01, 1:500) antibody was from Hypermol. Anti-penta-His (#34660, 1:2500) and anti-GST (#2622, 1:1000) antibodies were obtained from Qiagen and Cell Signaling, respectively. Antibodies against Cpn0677 were generated by Eurogentec (Belgium, 1:50 in immunofluorescence).
Secondary anti-rabbit, anti-rat and anti-mouse antibodies coupled to Alexa488 or Alexa594 (2 µg/ml) or coupled to alkaline phosphatase (1:10,000) were purchased from Thermo Scientific. Rhodamine-Phalloidin (#R415) was purchased from Thermo Scientific. All lipids used in this study were obtained from Avanti Lipids, NHS-FITC and NHS-Rhodamine and MitoTracker™-Red from Thermo Scientific. Wiskostatin (W2270-5MG) was purchased from Merck. G-actin (#8101-01), G-actin-Atto647 (#8158-03) and Arp2/3 (#8413-01) were obtained from Hypermol.
Spona D., Hanisch P.T., Hegemann J.H, & Mölleken K. (2023). A single chlamydial protein reshapes the plasma membrane and serves as recruiting platform for central endocytic effector proteins. Communications Biology, 6, 520.
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2

Antibody Sources and Reagents for Protein Analysis

Cited 1 time
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The primary antibody against SNX9 (OTI1E4) was purchased from Origene, anti-EGFR (PA1-1110) and anti-GFP (MA5-15256) were from Thermo Scientific, antipenta-His (#34660) from Qiagen, anti-GST (#2622) from Cell Signaling, and anti-DsRed (sc-101526) from Santa Cruz. Anti-IncA was a gift from G. Zhong, University of Texas Health Science Center at San Antonio, San Antonio, TX (57 (link)), and anti-DnaK was obtained from S. Birkelund, Aalborg University, Aalborg, Denmark (58 (link)). Antibodies against Cpn0677 and Cpn0678 were generated in our laboratory, as was anti-CPn0147. Mouse anti-Cya, rabbit anti-CRP, and rabbit anti-IpaD antibodies were generously donated by N. Guiso, A. Ullmann, and C. Parsot, Institut Pasteur, Paris, France, respectively. Secondary anti-rabbit and anti-mouse antibodies coupled to Alexa488 or Alexa594 were purchased from Thermo Scientific, and those coupled to alkaline phosphatase were sourced from Promega. CellMask (orange) and Rhodamine-Phalloidin were purchased from Thermo Scientific, and SiR-Tubulin from Spirochrome. All lipids used in this study were obtained from Avanti Lipids, and Texas red dye, NHS-FITC and DyLight650NHS from Thermo Scientific. Nocodazole and Cytochalasin D were purchased from Merck.
Hänsch S., Spona D., Murra G., Köhrer K., Subtil A., Furtado A.R., Lichtenthaler S.F., Dislich B., Mölleken K, & Hegemann J.H. (2020). Chlamydia-induced curvature of the host-cell plasma membrane is required for infection. Proceedings of the National Academy of Sciences of the United States of America, 117(5), 2634-2644.
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