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Isocage p bioexclusion system

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The ISOcage P - Bioexclusion System is a laboratory equipment designed to provide a contained and controlled environment for animal housing. It functions by creating a physical barrier to prevent the entry or exit of microorganisms, ensuring a high level of biosecurity and environmental isolation for the animals within.

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Lab products found in correlation

C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Female syrian hamsters, by Janvier Labs (1 mentions)

Close spelling variants of this product

Isocages (17 citations) ISOcage P (4 citations) Isocage system (4 citations) IsoCage bioexclusion systems (2 citations) Isocages bioexclusion system (2 citations)

11 protocols using isocage p bioexclusion system

1

Germ-Free Mouse Breeding and Maintenance

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All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Florida (UF) and performed at UF Animal Care Facilities (IACUC Protocol #201909606). Colonies of germ-free mice were bred and maintained in isolators by UF Animal Care Services Germfree Division. Mixed gender germ-free wild-type (GF-WT) C57BL/6 mice were transferred from breeding isolators and placed into the Techniplast ISOcage P Bioexclusion system to allow for microbial manipulation [7 (link), 8 (link)]. Cages were sterilely changed every 2 weeks per Techniplast protocol and supplied with autoclaved food and water.
Newsome R.C., Gharaibeh R.Z., Pierce C.M., da Silva W.V., Paul S., Hogue S.R., Yu Q., Antonia S., Conejo-Garcia J.R., Robinson L.A, & Jobin C. (2022). Interaction of bacterial genera associated with therapeutic response to immune checkpoint PD-1 blockade in a United States cohort. Genome Medicine, 14, 35.
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2

Evaluating Probiotic Impacts on Colon Cytokines

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C57Bl/6 mice were obtained from Jackson Laboratories and maintained in specific-pathogen free conditions inside Emory’s Gnotobiotic Animal Core (Atlanta, GA). Mice were orally gavaged every day with LGG, BC, or HBSS for 2 weeks, and kept inside an ISOcageP Bioexclusion system (Techniplast, West Chester, PA) maintained in a Biosafetly level 2 (BSL-2) facility. Upon killing, flash-frozen whole colons were given to RayBiotech, Inc (Peachtree Corners, GA) for protein extraction and cytokine analysis using the Quantibody Mouse Cytokine Array Q4000 (Raybiotech).
Owens J.A., Saeedi B.J., Naudin C.R., Hunter-Chang S., Barbian M.E., Eboka R.U., Askew L., Darby T.M., Robinson B.S, & Jones R.M. (2021). Lactobacillus rhamnosus GG Orchestrates an Antitumor Immune Response. Cellular and Molecular Gastroenterology and Hepatology, 12(4), 1311-1327.
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3

Hamster Model for Viral Infection

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Three-week-old female Syrian hamsters were provided by Janvier Labs. Animals were maintained in ISOcage P - Bioexclusion System (Techniplast) with unlimited access to water/food and 14 h/10 h light/dark cycle. Animals were weighed and monitored daily for the duration of the study to detect the appearance of any clinical signs of illness/suffering. Virus inoculation was performed under general anesthesia (isoflurane). Organs and blood were collected after euthanasia (cervical dislocation) which was also realized under general anesthesia (isoflurane).
Driouich J.S., Cochin M., Lingas G., Moureau G., Touret F., Petit P.R., Piorkowski G., Barthélémy K., Laprie C., Coutard B., Guedj J., de Lamballerie X., Solas C, & Nougairède A. (2021). Favipiravir antiviral efficacy against SARS-CoV-2 in a hamster model. Nature Communications, 12, 1735.
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4

Hamster Husbandry and Monitoring

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Three-week-old female golden Syrian hamsters were provided by Janvier Labs. Animals were maintained in ISOcage P - Bioexclusion System (Techniplast) with unlimited access to water/food and 14 h/10 h light/dark cycle. Animals were weighed and monitored daily for the duration of the study to detect the appearance of any clinical signs of illness/suffering.
Cochin M., Touret F., Driouich J.S., Moureau G., Petit P.R., Laprie C., Solas C., de Lamballerie X, & Nougairède A. (2021). Hydroxychloroquine and azithromycin used alone or combined are not effective against SARS-CoV-2 ex vivo and in a hamster model. Antiviral Research, 197, 105212.
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5

Syrian Hamster Care and Handling

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Three-week-old female Syrian hamsters were provided by Janvier Labs (SPF status). Animals were maintained in ISOcage P - Bioexclusion System (Techniplast) with unlimited access to water/food and 14h/10h light/dark cycle. A wooden gnawing block and extra bedding material was provided as cage enrichment. Animals were weighed and monitored daily for the duration of the study to detect the appearance of any clinical signs of illness/suffering. General anesthesia was obtained with isoflurane (Isoflurin®, Axience). Euthanasia, which was also realized under general anesthesia, was performed by cervical dislocation.
Driouich J.S., Cochin M., Touret F., Petit P.R., Gilles M., Moureau G., Barthélémy K., Laprie C., Wattanakul T., Chotsiri P., Hoglund R.M., Tarning J., Fraisse L., Sjö P., Mowbray C.E., Escudié F., Scandale I., Chatelain E., de Lamballerie X., Solas C, & Nougairède A. (2022). Pre-clinical evaluation of antiviral activity of nitazoxanide against SARS-CoV-2. eBioMedicine, 82, 104148.
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6

Tracking Hamster Illness During Viral Infection

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Female Syrian hamsters aged 3 weeks were provided by Janvier Labs. Animals were maintained in ISOcage P ‐ Bioexclusion System (Techniplast) with unlimited access to water/food and 14 h/10 h light/dark cycle. Every day, animals were weighed and monitored for the duration of the study to detect the appearance of any clinical signs of illness/suffering. Virus inoculation was performed under general anesthesia (isoflurane). Lungs and blood were collected after euthanasia (cervical dislocation) which was also carried out under general anesthesia (isofluorane).
Mélade J., Piorkowski G., Touret F., Fourié T., Driouich J., Cochin M., Bouzidi H.S., Coutard B., Nougairède A, & de Lamballerie X. (2022). A simple reverse genetics method to generate recombinant coronaviruses. EMBO Reports, 23(5), e53820.
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7

Cecal Transfer Modulates Colorectal Tumorigenesis

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Approximately 50 mg of cecal contents from each mouse was pooled from NC101-colonized DSS/Apcmin/+ mice treated with PBS or anti-TNF antibody in a type B vinyl anaerobic chamber (Coy Laboratory, 032714), resuspended in 10 ml of reduced sterile PBS and kept in sealed tubes on ice until gavage. Germ-free Apcmin/+ mice (12–18 weeks old) were transferred from breeding isolators to individually ventilated cages on the IsoCage P Bioexclusion System (Techniplast) and supplied with autoclaved water and food. After 1-week acclimation, mice were inoculated with 200 μl of freshly prepared cecal content slurry by single oral gavage. At 10 d after gavage, mice received 1.5% DSS (molecular weight 40 kDa, Alfa Aesar, J63606; filter-sterilized) for 1 week. Mice were then kept on autoclaved regular drinking water for 4 weeks before euthanasia. Stools and cecal contents were collected using sterile tools at indicated time points.
Yang Y., Gharaibeh R.Z., Newsome R.C, & Jobin C. (2020). Amending microbiota by targeting intestinal inflammation with TNF blockade attenuates development of colorectal cancer. Nature cancer, 1(7), 723-734.
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8

Animal Welfare in Virus Research

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Animals were maintained in an ISOcage P Bioexclusion System (Techniplast) with unlimited access to food and water and 12 h light/12 h dark cycle. Animals were individually monitored every day to detect the appearance of any clinical sign of illness/suffering. Virus/Antibody inoculation, blood collection, and euthanasia (cervical dislocation) were performed under general anesthesia (isofluorane).
Touret F., Gilles M., Klitting R., Aubry F., de Lamballerie X, & Nougairède A. (2018). Live Zika virus chimeric vaccine candidate based on a yellow fever 17-D attenuated backbone. Emerging Microbes & Infections, 7, 161.
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9

Golden Syrian Hamster Care Protocol

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Three-week-old female golden Syrian hamsters were provided by Janvier Labs. Animals were maintained in ISOcage P - Bioexclusion System (Techniplast) with unlimited access to water/food and 14h/10h light/dark cycle. Animals were weighed and monitored daily for the duration of the study to detect the appearance of any clinical signs of illness/suffering.
Touret F., Driouich J.S., Cochin M., Petit P.R., Gilles M., Barthélémy K., Moureau G., Mahon F.X., Malvy D., Solas C., de Lamballerie X, & Nougairède A. (2021). Preclinical evaluation of Imatinib does not support its use as an antiviral drug against SARS-CoV-2. Antiviral Research, 193, 105137.
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10

Hamster Model for Viral Infection

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Female Syrian hamsters aged three-weeks-old were provided by Janvier Labs. Animals were maintained in ISOcage P -Bioexclusion System (Techniplast) with unlimited access to water/food and 14h/10h light/dark cycle. Every day, animals were weighed and monitored for the duration of the study to detect the appearance of any clinical signs of illness/suffering. Virus inoculation was performed under general anesthesia (isoflurane). Lungs and blood were collected after euthanasia (cervical dislocation) which was also realized under general anesthesia (isofluorane).
Mélade J., Piorkowski G., Touret F., Fourié T., Coutard B., Nougairède A., & Lamballerie X.d. (2021). Infectious Subgenomic Amplicons method to expedite reverse genetics of SARS-CoV-2 and other coronaviruses.
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