The expression of ACE2/TMPRSS2 was analyzed by using the anti-ACE2 (535919) from R&D, and the anti-TMPRSS2 (EPR3862) from abcam according to our methods (28 (link)–30 (link)). Besides, Live/dead fixable dead cell stain (ThermoFisher) was used to exclude dead cells in flow cytometry. Paraformaldehyde fixed cells were acquired by flow cytometry using a LSRFORTESSA flow cytometer (BD) and analyzed with FlowJo software (version 10).
Anti ace2 535919
Manufactured by R&D Systems
Anti-ACE2 (535919) is a recombinant human protein that binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor. This protein can be used in research applications to study the interaction between ACE2 and its binding partners.
Automatically generated - may contain errors
3 protocols using anti ace2 535919
1
Quantifying ACE2/TMPRSS2 Expression by Flow Cytometry
2
Phenotyping Human Cell Antigens
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fluorophore antibodies with specificity to human cell surface antigens were purchased mainly from BD Biosciences or Thermo Fisher Scientific. Specifically, the following antibodies were used: anti-CD3 (HIT3a), anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD45 (H-130 or 2D1), anti-CD107a (H4A3), anti–Granzyme B (GzmB; GB11), anti-CD71 (MA712), anti-CD235A (HIR2), and arginase I (IC5868N/R&D). In addition, anti-ACE2 (535919) from R&D, anti-TMPRSS2 (EPR3862) from abcam, and DCFDA (Sigma) were used for staining. Besides, live/dead fixable dead cell stains (Thermo Fisher) were used to exclude dead cells in flow cytometry. Paraformaldehyde fixed cells were acquired by flow cytometry using an LSRFORTESSA flow cytometer (BD) and analyzed with FlowJo software.
3
Phenotyping Human Cell Antigens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorophore antibodies with specificity to human cell surface antigens were purchased mainly from BD Biosciences or Thermo Fisher Scientific. Specifically, the following antibodies were used: anti-CD3 (HIT3a), anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD45 (H-130 or 2D1), anti-CD107a (H4A3), anti–Granzyme B (GzmB; GB11), anti-CD71 (MA712), anti-CD235A (HIR2), and arginase I (IC5868N/R&D). In addition, anti-ACE2 (535919) from R&D, anti-TMPRSS2 (EPR3862) from abcam, and DCFDA (Sigma) were used for staining. Besides, live/dead fixable dead cell stains (Thermo Fisher) were used to exclude dead cells in flow cytometry. Paraformaldehyde fixed cells were acquired by flow cytometry using an LSRFORTESSA flow cytometer (BD) and analyzed with FlowJo software.
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