Fibronectin coated plates

Manufactured by Corning

Sourced in Germany, United States

Fibronectin-coated plates are a type of lab equipment designed for cell culture applications. They provide a specialized surface that promotes cell adhesion and proliferation. The fibronectin coating mimics the extracellular matrix, facilitating cell attachment and growth. These plates are intended for use in various research and experimentation processes involving cell-based studies.

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Lab products found in correlation

Cell observer hs, by Zeiss (1 mentions) Zen software, by Zeiss (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Penicillin streptomycin, by Beyotime (1 mentions) Hepatocyte wash medium, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Hepg2 cell, by American Type Culture Collection (1 mentions) Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (1 mentions) F12 medium, by Fujifilm (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Its x, by Thermo Fisher Scientific (1 mentions)

4 protocols using fibronectin coated plates

Zebrafish Cell Migration Assay

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Cell
migration was monitored as wound healing assay on a Zeiss Cell Observer
HS widefield microscope with a 10× objective. A total of 9 ×
10⁵ cells mL^–1 of the respective HEK293
stable cell lines were seeded into fibronectin-coated plates (Corning)
and allowed to settle for 20 h in the presence of tetracycline. The
plates were manually equipped with migration inserts for self-insertion
(Ibidi) by placing the insert with sterile tweezers and gently pressing
it onto the plate. Cell culture dishes and matrix component-precoated
plates were used according to the information in the text. On the
day of experimentation, new growth medium was added containing 7.5%
(v/v) FBS (Sigma), and the inserts were gently removed with sterile
tweezers. Dishes were kept at 37 °C, and pictures of the wound
gap were taken at 0 and 1.5 h time points, using Zeiss ZEN software.
All pictures were analyzed with Fiji software according to length
(unit) difference of the remaining wound gap and presented as speed
of migration (unit/h). Independent experiments were performed at least
in triplicate.

Hoeger B., Rios P., Berteotti A., Hoermann B., Duan G, & Köhn M. (2017). Mutational Analysis of a Conserved Glutamate Reveals Unique Mechanistic and Structural Features of the Phosphatase PRL-3. ACS Omega, 2(12), 9171-9180.

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Isolation of CD133+ Cells from Proliferating IH Tissues

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The proliferating IH tissues resected from the patients were immediately immersed in a growth medium at 4°C [Dulbecco's modified Eagle's medium, high glucose, 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit Haemek, Israel), and 1% penicillin-streptomycin (PS; Beyotime Institute of Biotechnology, Haimen, China)] and then quickly taken to our laboratory. The fatty and skin tissue were resected, the samples were rinsed three times in phosphate-buffered saline (PBS) and then minced. A 0.2% compound of collagenase (cat. no. 17454; Serva Electrophoresis GmbH, Heidelberg, Germany) was used to digest the samples at 37°C for 2 h until they were chylous. They were then filtered through a 100-micron cell strainer. From this single cell suspension, cells expressing CD133 were selected using a magnetic beads technology (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then cultured on fibronectin-coated plates (Corning Incorporated, Corning, NY, USA) in EGM-2 media (cat. no. CC-4176; Lonza Group, Ltd., Basel, Switzerland) that was supplemented by 20% FBS and 1% PS.

Zhang K., Wang F., Huang J., Lou Y., Xie J., Li H., Cao D, & Huang X. (2018). Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells. Experimental and Therapeutic Medicine, 17(3), 1663-1669.

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Isolation and Hypoxia Induction of Hepatic Cell Lines

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HepG2 cells (HB‐8065; ATCC, Manassas, VA, USA) were grown in Minimum Essential Medium (Invitrogen, USA), supplemented with 10% foetal bovine serum (Invitrogen), 100 mg/ml streptomycin, 100 IU/ml penicillin and 2 mM L‐glutamine. Primary hepatocytes were isolated and cultured from mice as previously described.²⁷ Briefly, after mice were anesthetised, their livers were perfused via the portal vein with freshly prepared perfusion medium (Invitrogen), followed by digestion buffer (0.33 mg of collagenase I/ml). The livers were subsequently dispersed with hepatocyte wash medium (Invitrogen), and then seeded onto fibronectin‐coated plates (Corning, USA). For hypoxia induction in vitro, cells were incubated in an airtight chamber in an atmosphere of 1% O₂, 94% N₂ and 5% CO₂ for the time periods indicated.

Wang C., Zhang W., Xu W., Liu Z, & Huang K. (2022). AMP‐activated protein kinase α1 phosphorylates PHD2 to maintain systemic iron homeostasis. Clinical and Translational Medicine, 12(5), e854.

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Ex vivo Expansion of Hematopoietic Stem Cells

Cited 1 time

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For ex vivo culture, CD34^-CD150⁺CD48^-LSK cells were isolated from femurs and tibiae of C57BL/6-CD45.2⁺ mice by flow cytometry, plated into flat-bottomed 96-well fibronectin-coated plates (Corning; 354409) at 50 cells per well in medium composed of F12 medium (Wako), 1% ITSX (Gibco), 10 mM HEPES (Gibco), 1% P/S/G (Gibco), 100 ng/ml mouse TPO (Wako), 10 ng/ml mouse SCF, 0.1% PVA (Peprotech) with or without 100 ng/ml mouse CXCL12 (Kola-Gen Pharma), and cultured at 37 °C with 5% CO₂ for 28 days. Medium changes were performed as described previously^{30 (link)}. Following ex vivo culture, 25% of the cells were prepared for flow cytometric analysis and 75% of the cells were prepared for competitive repopulation analysis from each well. Flow cytometric analysis and competitive repopulation analysis were performed as described above.

Nakatani T., Sugiyama T., Omatsu Y., Watanabe H., Kondoh G, & Nagasawa T. (2023). Ebf3+ niche-derived CXCL12 is required for the localization and maintenance of hematopoietic stem cells. Nature Communications, 14, 6402.

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