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4 protocols using lactic acid

1

Baking Studies Using Yeast Extracts

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The used chemicals were hydrochloric acid, nitric acid, and lactic acid from Scharlau (Scharlab S.L., Spain), and iron nitrate, ascorbic acid, and 70% ethanol (Sigma Aldrich, Stockholm, Sweden). The deionized water was generated by Millipore Milli‐Q plus ultrapure water system (Millipore, Solna, Sweden). The four yeast extracts (YE1 to YE4) used for the baking studies were as follows: YE1 (Acros organics from Fisher Scientific, UK), YE2 (Bacto yeast extract from Difco Laboratories, UK), YE3 (Oxoid yeast extract from Oxoid Ltd, UK), and YE4 (yeast extract from Sigma Aldrich, Stockholm, Sweden).
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2

Corrosion Inhibition of Carbon Steel

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Magnesium chloride hexahydrate (MgCl2·6H2O, 98%), aluminum chloride hexahydrate (AlCl3·6H2O, 99%), and sodium chloride (NaCl 99%) were obtained from Alfa Aesar. Sodium hydroxide (NaOH, 98%), hydrochloric acid (HCl, 36.5%), and lactic acid (C3H6O3, 88%) were purchased from Scharlau. Organic inhibitor 2-benzothiazolythio-succinic acid (BTSA 98.5%) was received from Zhengzhou HQ Material Company. All the chemicals were used as received.
The S45C carbon steel electrodes with a surface area of 1.13 cm2 were used to prepare the working electrode. For all experiments, the electrode surface was polished with SiC abrasive paper (Fujistar-Japan) from 300 to 1200 grades, rinsed with ethanol, and dried before testing. The surface compositions of electrodes after polishing were 3.14 wt% C, 0.98 wt% O, 0.08 wt% Si, 0.78 wt% Mn, 1.28 wt% S, 0.05 wt% P, 0.18 wt% Cr, 0.09 wt% Ni and the rest is Fe.
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3

Synthesis of Epoxy-Based Insulation Materials

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Aluminum chloride hexahydrate
(AlCl3.6H2O, 99%), and magnesium chloride hexahydrate
(MgCl2·6H2O, 98%) were obtained from Alfa
Aesar. Sodium hydroxide (NaOH, 98%), sodium carbonate (Na2CO3, 99.5%), hydrochloric acid (HCl 36.5%), and lactic
acid (C3H6O3, 88%) were purchased
from Scharlau. A commercial grade of Na2SiO3 (sodium silicate solution Na2O 9–10%, SiO2 26–28%) was obtained from Vinachem.
The insulation
film base on the epoxy system, which included epoxy bisphenol A (D.E.R-331)
and polyamine hardener (XUS 19036.00) were purchased from Dow Chemical.
Industrial-grade of solvent used for dilution as methyl isobutyl ketone
(MIBK) was purchased from Kumho P&B Chemicals (Korea). All the
chemicals were used as received.
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4

Fabrication of Polysaccharide Nanofilms

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Quartz slides (suprasil ® 300) were purchased from Hellma analytics (Müllhein, 82 Germany) and polyethylene terephthalate (PET) sheets were obtained from Isovolta 83 (Barcelona, Spain). Reactives for substrate aminolization such as, 3-84 aminopropyltriethoxysilane (APTS, 99 %), N,N-Dimethylformamide (DMF, 99,8 %) 85 and 1,6-Hexanediamine (99,5 %) were purchased from Acros Organics (Geel, 86 Belgium). Piperidine (99 %) was purchased from Sigma-Aldrich (Madrid, Spain). 87
Methanol and bromophenol blue (BPB) were obtained from Fisher Scientific 88 (Loughborough, UK). Ethanol Absolute and 1-propanol were purchased from Scharlau 89 (Barcelona, Spain). Hydroclorhidric acid (HCl, 35-38%) was purchased from POCH 90 (Gliwice, Poland). Chitosan (CHI, high molecular weight, deacetylation degree >75%) 91 and sodium alginate (ALG; manucol ® DH) were purchased from Sigma-Aldrich 92 (Madrid, Spain) and FMC biopolymers (Scotland, UK), respectively. The pH of the 93 polysaccharide solutions was adjusted with lactic acid (88-90%) and sodium hydroxide 94 (NaOH) purchased from Scharlau (Barcelona, Spain). Deionized water (18,2 mΩ, Milli-95 Q ultrapure water system, Millipore) was used in all film-forming solutions and 96 washing steps for the nanofilms fabrication. 97
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