Mass spectroscopy was conducted to assess infarct size by quantifying gadolinium in the scar. ICP-MS was performed on scar tissue 7 days and 30 days after permanent ligation. The model ELAN DRCplus (Perkin Elmer, Waltham, Massachusetts, US) was used with the software package Perkin Elmer’s Elan v. 3.3. Principal settings were plasma, auxiliary, and nebulizer gas flows 15, 0.8, and 0.97 l/min of argon with an RF power of 1200 W. The sample flow rate was 0.4 ml/min. The residence times on gadolinium were averaged over five replicate readings (data not shown).
Elan drcplus
Manufactured by PerkinElmer
Sourced in United States, Canada
The ELAN DRCplus is a high-performance inductively coupled plasma mass spectrometer (ICP-MS) instrument designed for accurate and sensitive elemental analysis. It offers advanced features for trace-level detection and quantification of a wide range of elements in various sample types.
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Lab products found in correlation
4 protocols using elan drcplus
1
Quantifying Myocardial Infarct Size via ICP-MS
2
Analytical Characterization of Compound 4
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NMR analyses
were performed with a 500 MHz Bruker AVANCE-III NMR-system. The multiplicities
are abbreviated as follows: s = singlet, d = doublet, t = triplet,
m = multiplet, br = broad signal, dd = doublet of doublets, etc. MS
analyses were performed with a linear ion trap quadrupole mass spectrometer
(QTRAP, Applied Biosystems SCIEX) equipped with a Turbo Ion Spray
source. Purity of the reference standard4 was determined
with HPLC. Ruthenium content in the final preparation was analyzed
with inductively coupled plasma mass spectrometry (ICP-MS; PerkinElmer,
Elan DRC Plus). Commercial standards were used for instrument calibration.
were performed with a 500 MHz Bruker AVANCE-III NMR-system. The multiplicities
are abbreviated as follows: s = singlet, d = doublet, t = triplet,
m = multiplet, br = broad signal, dd = doublet of doublets, etc. MS
analyses were performed with a linear ion trap quadrupole mass spectrometer
(QTRAP, Applied Biosystems SCIEX) equipped with a Turbo Ion Spray
source. Purity of the reference standard
with HPLC. Ruthenium content in the final preparation was analyzed
with inductively coupled plasma mass spectrometry (ICP-MS; PerkinElmer,
Elan DRC Plus). Commercial standards were used for instrument calibration.
3
Urinary Arsenic Speciation Analysis
4
Elemental Gadolinium Bioaccumulation Analysis
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Elemental Gd was assayed in the plasma, urine, feces, spleen, heart, brain (cerebral cortex), cerebellum, dorsal skin, liver (1 sample from the left lobe), left kidney, and left femur (both epiphyses) by inductively coupled plasma mass spectrometry (ICP-MS) using an ELAN DRC Plus (PerkinElmer Life and Analytical Sciences, Inc, Waltham, MA) after sample mineralization in 65% nitric acid for 8 hours at a temperature of 80°C. The lower limit of quantification in diluted mineralized solutions was 0.05 μg/L.
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