Peripheral blood was collected from mice treated with vehicle or clodronate liposomes and 50 µL of each sample was spiked with re-fluorescent beads (Invitrogen, CA) as internal standards for absolute counts. Red blood cells were lysed with red blood cell lysis buffer (BioLegend, San Diego, CA), and mononuclear cells (MNCs) were isolated. MNCs were then washed with cold PBS and stained with CD11b-fluorescein isothiocyanate (FITC) and CD45-phycoerythrin (PE) (BD Pharmingen, BD Biosciences, San Jose, CA) for 1 hr on ice. Then, cells were washed, re-suspended in PBS and analyzed by flow cytometry. Forward scatter (FSC) and side scatter (SSC) were used to gate monocytes as high-size (FSC)/ low-granulation (SSC) population; moreover, the monocyte population was further characterized as CD11b-positive/CD45-positive. The count of the FSC-high/SSC-low/CD11b-positive/CD45-positive monocyte population was normalized to the count of the fluorescent beads. Results were presented as the % of average of vehicle treated mice.
Re fluorescent beads
Manufactured by Thermo Fisher Scientific
Sourced in Canada
Re-fluorescent beads are a type of laboratory equipment used for various applications. They consist of polystyrene or silica beads that have been embedded with fluorescent dyes. These beads can be excited by light, causing them to emit a specific wavelength of light, which can be detected and measured.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using re fluorescent beads
1
Quantifying Monocyte Populations in Mice
2
Quantifying Monocyte Populations in Mice
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Peripheral blood was collected from mice treated with vehicle or clodronate liposomes and 50 µL of each sample was spiked with re-fluorescent beads (Invitrogen, CA) as internal standards for absolute counts. Red blood cells were lysed with red blood cell lysis buffer (BioLegend, San Diego, CA), and mononuclear cells (MNCs) were isolated. MNCs were then washed with cold PBS and stained with CD11b-fluorescein isothiocyanate (FITC) and CD45-phycoerythrin (PE) (BD Pharmingen, BD Biosciences, San Jose, CA) for 1 hr on ice. Then, cells were washed, re-suspended in PBS and analyzed by flow cytometry. Forward scatter (FSC) and side scatter (SSC) were used to gate monocytes as high-size (FSC)/ low-granulation (SSC) population; moreover, the monocyte population was further characterized as CD11b-positive/CD45-positive. The count of the FSC-high/SSC-low/CD11b-positive/CD45-positive monocyte population was normalized to the count of the fluorescent beads. Results were presented as the % of average of vehicle treated mice.
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