Proliferation of transfected HEK293 cells (as determined by DNA synthesis) was measured by a colorimetric bromodeoxyuridine (BrdU) assay kit from Roche (Mannheim, Germany) according to the manufacturer's protocol. Transfected HEK293 cells were seeded in a 96-well plate coated with collagen I or PBS (as a control) (2 × 104 cells/well). The BrdU reagent was added for 2 h according to the provided protocol. Proliferation was measured by light absorbance at 370/492 nm.
Colorimetric
Manufactured by Roche
Sourced in Germany
Colorimetric is a type of lab equipment used to measure the concentration of a substance in a solution by analyzing the color intensity of the solution. It works by passing light through a sample and measuring the amount of light absorbed, which is proportional to the concentration of the substance being measured.
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Lab products found in correlation
2 protocols using colorimetric
1
Measuring HEK293 Cell Proliferation
2
Quantifying Cellular Proliferation in Hypoxia
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The proliferation ratio was determined in 3 biological replicates, with Cell Proliferation ELISA, BrdU, and colorimetric (#11 647 229 001, Roche, Basel, Switzerland), according to the manufacturer’s protocol. Briefly, 5000 cells were seeded in a 96-well plate in 100 µL/well and incubated at hypoxic conditions at 37 °C for 48 h. BrdU labeling was performed for 2 h. Cells were incubated with the anti-BrdU solution for 90 min and then with a substrate solution for 10 min. The absorbance was measured at 370 nm and calculated by subtracting the blank control absorbance value. Statistical results were calculated in GraphPad Prism6 (RRID:SCR_002798, GraphPad Software). One-way ANOVA variance analysis with a post-hoc Dunnett’s test was conducted.
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