Fluorescent samples were imaged using an inverted fully automated confocal Nikon AR-1 microscope. The NIS elements software was used for image acquisition/elaboration.
Ar 1 microscope
Manufactured by Nikon
The AR-1 microscope is a high-quality laboratory instrument designed for detailed observation and analysis. It features a stable optical system, precise focusing mechanism, and LED illumination for clear, detailed images. The AR-1 is suitable for a variety of scientific and research applications that require close examination of samples.
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Lab products found in correlation
2 protocols using ar 1 microscope
1
Confocal Imaging of Fluorescent Samples
2
Nanoparticle Internalization in Neural-Differentiated ESCs
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For nanoparticles
internalization experiments, neural-differentiated ESCs were seeded
in six-well plates and cultured for 24 h. Cu0–AuNPs
or L1@Cu2+–AuNPs conjugated with Alexa-Fluor-594,
suspended in culture medium at 200 nM, were incubated with cells for
24 h. After the incubation, the cells were rinsed twice in PBS to
remove the noninternalized nanoparticles, fixed in 4% paraformaldehyde
for 30 min, and washed in PBS 1×. After washing, the cells were
incubated with WGA-488 (Invitrogen) as membrane marker following manufacturer’s
instructions. Nuclei were counterstained with Hoechst 33342 (Invitrogen).
Fluorescent labeling was visualized using the inverted fully automated
confocal Nikon AR-1 microscope. The NIS elements software was used
for image acquisition/elaboration.
internalization experiments, neural-differentiated ESCs were seeded
in six-well plates and cultured for 24 h. Cu0–AuNPs
or L1@Cu2+–AuNPs conjugated with Alexa-Fluor-594,
suspended in culture medium at 200 nM, were incubated with cells for
24 h. After the incubation, the cells were rinsed twice in PBS to
remove the noninternalized nanoparticles, fixed in 4% paraformaldehyde
for 30 min, and washed in PBS 1×. After washing, the cells were
incubated with WGA-488 (Invitrogen) as membrane marker following manufacturer’s
instructions. Nuclei were counterstained with Hoechst 33342 (Invitrogen).
Fluorescent labeling was visualized using the inverted fully automated
confocal Nikon AR-1 microscope. The NIS elements software was used
for image acquisition/elaboration.
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