Sf001 500p

Manufactured by Merck Group

The SF001-500P is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of the SF001-500P is to provide a controlled environment for various experiments and research activities.

Automatically generated - may contain errors

Lab products found in correlation

4 hydroxytamoxifen, by Merck Group (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Esg1107, by Merck Group (1 mentions) M3148, by Merck Group (1 mentions) B27 without vitamin a, by Thermo Fisher Scientific (1 mentions)

3 protocols using sf001 500p

Derivation and Characterization of Reciprocal Hybrid ES Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The derivation and characterization of the reciprocal hybrid ES cell lines between the M. m. domesticus strain C57BL/6J and the M. m. molossinus strain JF1 (lines BJ1 and JB1, respectively) are described in Kotaet al. (39 (link)). These ES cell lines are maintained on gelatin-coated dishes in ESGRO-complete-plus medium (Millipore, SF001-500P) that contains LIF and BMP4.
DNA and RNA samples from the B1.3 Eed^−/− and WT ES cell lines (26 (link)) were from Amanda Fisher's laboratory (Lymphocyte Development Group, MRC, London, UK).
MEF cells were isolated from E13.5 Ezh2 flox/flox;ROSA26-CreERT2 mouse embryos and then infected with the pBABE-hygro p53-DD retroviral construct (addgene 9058) to generate p53-DN immortalized MEFs (iMEFs). iMEFs were grown in Dulbecco's modified Eagle's medium (Gibco, 11960-044) with 10% fetal bovine serum (PAA, A15-101), 2 mM glutamine (PAA M11-006) and 1% MEM Non-essential amino acids solution (Gibco 11140-035) at 37°C in 5% CO2. For conditional Ezh2 deletion, iMEFs were incubated with 4-hydroxytamoxifen (Sigma, T176) at a final concentration of 1 μM.

Maupetit-Méhouas S., Montibus B., Nury D., Tayama C., Wassef M., Kota S.K., Fogli A., Cerqueira Campos F., Hata K., Feil R., Margueron R., Nakabayashi K., Court F, & Arnaud P. (2015). Imprinting control regions (ICRs) are marked by mono-allelic bivalent chromatin when transcriptionally inactive. Nucleic Acids Research, 44(2), 621-635.

+ Open protocol

+ Expand

Genetically Engineered mESC Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

E14 mouse embryonic stem cells (mESCs) were obtained from the American Type Culture Collection (ATCC). Genetically engineered mESCs, including Rif1-CKO, HA-Rif1 KI, HA-Pcgf6 KI, HA-Mga KI, and 2C::tdTomato reporter cell lines were constructed and cultured in gelatin-coated plates using high-glucose Dulbecco’s modified Eagle’s medium (DMEM, HyClone, SH3002202b) supplemented with 15% ESC-qualified fetal bovine serum (FBS, Vistech, SE200-ES), 0.1 mM 2-mercaptoethanol (Sigma, m3148), 1× non-essential amino acids (NEAA, Gibco, 11140050), and 1000 U/mL of LIF (Millipore, ESG1107). For maintenance, mESCs were cultured in the serum-free ESGRO medium (Millipore, sf001–500p). The U2OS-LacO cells (kindly provided by Professor Xuebiao Yao) and HEK293T cells were maintained in high-glucose DMEM supplemented with 10% FBS (Vistech, SE100-B). All cell lines were tested for mycoplasma contamination, and cultured at 37 °C in a humidified incubator containing 5% CO₂.

Li L., Li P., Chen J., Li L., Shen Y., Zhu Y., Liu J., Lv L., Mao S., Chen F., Hu G, & Yuan K. (2022). Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential. Cell Regeneration, 11, 25.

+ Open protocol

+ Expand

Hybrid Embryonic Stem Cell Corticogenesis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hybrid male embryonic stem cell (ESC) lines were previously derived from blastocysts obtained from crosses between C57BL/J (B) and JF1 (J) mice³⁴ (link) and were maintained in gelatin-coated dishes with ESGRO complete plus medium (Millipore, SF001-500P) containing LIF (leukemia inhibitory factor), BMP4 (bone morphogenetic protein 4), and a GSK3-β (glycogen synthase kinase 3β) inhibitor. In vitro corticogenesis was performed as previously described,³⁵ (link) except that ESCs were plated on Matrigel-coated dishes (human ESC-qualified matrix, Corning), and that the defined default medium was supplemented with B27 (without vitamin A, Gibco) to improve cell survival and with 1 μM dorsomorphin homolog 1 (purified by C.C.H.) to promote neurogenesis.³⁶ (link) Using this protocol, neural precursor cells (NPCs) are the main cell population after 12 days (D12) of in vitro corticogenesis.³⁵ (link)

Rengifo Rojas C., Cercy J., Perillous S., Gonthier-Guéret C., Montibus B., Maupetit-Méhouas S., Espinadel A., Dupré M., Hong C.C., Hata K., Nakabayashi K., Plagge A., Bouschet T., Arnaud P., Vaillant I, & Court F. (2024). Biallelic non-productive enhancer-promoter interactions precede imprinted expression of Kcnk9 during mouse neural commitment. Human Genetics and Genomics Advances, 5(2), 100271.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!