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Nestin percpcy5

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Nestin PerCPCy5.5 is a reagent used in flow cytometry applications. It is a fluorescently conjugated antibody that binds to the Nestin protein, which is a marker for neural stem and progenitor cells.

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Lab products found in correlation

Cd133 fitc, by BD (1 mentions) Sox2 fitc, by Thermo Fisher Scientific (1 mentions) Cd133 apc, by Miltenyi Biotec (1 mentions) Accutase, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Cd45 fitc, by BD (1 mentions) Paraformaldehyde (pfa), by Solarbio (1 mentions)

2 protocols using nestin percpcy5

1

Glioblastoma Tumor Cell Immunophenotyping

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Fluorescein-conjugated human antigen-specific or isotype control antibodies and anti-human IL-17RA PE were purchased from eBioscience; CD133 FITC and Nestin PerCPCy5.5 were from BD Bioscience, San Jose, CA; CD133 APC from Miltenyi Biotec; and Sox2 FITC was purchased from eBioscience. A published protocol was followed for surface and intracellular staining of the cells [79 (link)]. Tumor tissues obtained from 5 patients with histologically-verified glioblastoma were studied.
Parajuli P., Anand R., Mandalaparty C., Suryadevara R., Sriranga P.U., Michelhaugh S.K., Cazacu S., Finniss S., Thakur A., Lum L.G., Schalk D., Brodie C, & Mittal S. (2016). Preferential expression of functional IL-17R in glioma stem cells: potential role in self-renewal. Oncotarget, 7(5), 6121-6135.
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2

Characterizing Neural Stem Cells

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Single-cell suspensions were obtained from different passages of the neurosphere with Accutase (Invitrogen) for 5 min at 37 °C, then the cells were fixed with 4% paraformaldehyde (Solarbio) for 20 min. NESTIN-PerCP-Cy5.5 (BD Biosciences, Mountainview, CA, USA) and CD45-FITC (BD Biosciences) were incubated for 30 min at room temperature, shielded from light. The cell concentration was about 5 × 106 cells per milliliter of DPBS and the antibody dilution was used in accordance with the recommendations of the instructions. Cells were analyzed by the FACS Calibur with CellQuest software (BD Biosciences).
Liu B.C., Liu F.Y., Gao X.Y., Chen Y.L., Meng Q.Q., Song Y.L., Li X.H, & Bao S.Q. (2021). Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells. International Journal of Molecular Sciences, 22(14), 7473.
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