C. elegans were maintained on Escherichia coli strain OP50 on nematode growth medium (NGM) agar plates at 20°C. Punc-104::human Kif1a vectors were prepared as described (14 (link)). Four nanograms of Punc-104::human Kif1a and 50 ng of Podr-1::gfp vectors were injected to unc-104(e1265) gonads by standard procedures (52 (link)). At the F1 generation, worms with visible green fluorescent protein (GFP) signal in head neurons were collected. Stable transformation was confirmed by transmission of extrachromosomal arrays to the F2 generation. Functional complementation of unc-104 by human KIF1A was assessed by the motility of worms on NGM plates. Worms with visible markers were transferred to new plates, and movement was recorded under an SteREO Discovery.V12 dissection microscope (Carl Zeiss, Jena, Germany) equipped with Orca-Flash 2.8 CMOS camera (Hamamatsu Photonics, Hamamatsu, Japan).
Stereo discovery v12 dissection microscope
Manufactured by Zeiss
Sourced in Germany
The SteREO Discovery.V12 dissection microscope is a versatile and high-performance optical instrument designed for a variety of laboratory applications. It provides a wide field of view, high optical quality, and advanced features to support precise observation and analysis.
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Lab products found in correlation
2 protocols using stereo discovery v12 dissection microscope
1
Functional Complementation of unc-104 by human KIF1A in C. elegans
2
Functional Complementation of C. elegans UNC-104 by Human KIF1A
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C. elegans were maintained on E.coli strain OP50 on Nematode growth medium (NGM) agar plates at 20 °C. Punc-104::human Kif1a vectors were prepared as described (14) . 4ng of Punc-104::human Kif1a and 50 ng of Podr-1::gfp vectors were injected to unc-104(e1265) gonads by standard procedures (Mello et al., 1991) . At the F1 generation, worms with visible GFP signal in head neurons were collected. Stable transformation was confirmed by transmission of extrachromosomal arrays to the F2 generation. Functional complementation of unc-104 by human KIF1A was assessed by the motility of worms on NGM plates. Worms with visible markers were transferred to new plates and movement was recorded under an SteREO Discovery.V12 dissection microscope (Carl Zeiss, Jena, Germany) equipped with Orca-Flash 2.8 CMOS camera (Hamamatsu Photonics, Hamamatsu, Japan). Data were analyzed using Prism ver.7.
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