Cck 8 solution

In 96-well culture dishes, A549 cells were cultured for various lengths of time in a medium containing various concentrations of BLM, ARC, and ARC@DPBNPs. Each well (containing 100 L of media) received 10 µL of CCK-8 solution (Yesen, Shanghai, China), which was then cultured for 2 h at 37°C. Using a microplate reader (Type:1,510, Thermo Fisher Scientific), the absorbance of each group was determined at 450 nm (n = 5).
Ten microliters of CCK-8 solution (Yesen, Shanghai, China) were added to each well (containing 100 μL medium) and cultured for 2 h at 37°C. The absorbance of each group was measured at 450 nm (n = 5) using a microplate reader (Type:1,510, Thermo Fisher Scientific). The absorbance and number of live cells were directly proportionally related.

The treated neural stem cells (2 × 10⁴ cells/100 μL) were inoculated for 96 hours and 10 μL of CCK-8 solution (Ye Sen, Shanghai, China) was added to each sample, 3 replicate wells were repeated. After incubating for 2–4 hours, the absorbance at 450 nm was measured with a microplate reader (BD-Biosciences Franklin Lakes, NJ, USA).