Molecular loading into the
HEWL crystals was performed using the following procedure. The sealing
film was initially peeled off from the crystallization plate, and
7 μL of various concentrations of the dye in a sodium acetate
buffer solution was added to the well containing HEWL crystals. Subsequently,
the plate was sealed again and placed under an inverted microscope
for optical measurement. Notably, HEWL was mixed with the dye solution
(50 mg/mL) to avoid crystal dissolution, owing to decreasing concentration.
The dye types employed in this study are provided inChart 1 . Acridine yellow G was purchased
from Sigma-Aldrich, whereas eosin Y and rhodamine 6G were obtained
from Wako Chemical and used without further purification.
HEWL crystals was performed using the following procedure. The sealing
film was initially peeled off from the crystallization plate, and
7 μL of various concentrations of the dye in a sodium acetate
buffer solution was added to the well containing HEWL crystals. Subsequently,
the plate was sealed again and placed under an inverted microscope
for optical measurement. Notably, HEWL was mixed with the dye solution
(50 mg/mL) to avoid crystal dissolution, owing to decreasing concentration.
The dye types employed in this study are provided in
from Sigma-Aldrich, whereas eosin Y and rhodamine 6G were obtained
from Wako Chemical and used without further purification.