C57BL/6J (B6) mice were purchased from the Beijing Vital River Laboratory Animal Technology Co. Ltd. Female C57BL/6J (B6) mice (20–25 g) were housed in a temperature-controlled environment at 23–25 °C with a 12/12 h light/dark cycle and were given access to food and water without restriction. All care and handling of mice were performed according to the standard guidelines for the care and use of experimental animals in Chinese Academy of Medical Sciences&Peking Union Medical College Institute of Radiation Medicine.
C57bl 6j b6 mice
Manufactured by Charles River Laboratories
Sourced in United States, Japan
The C57BL/6J (B6) mice are a commonly used inbred mouse strain. They are a widely utilized model organism for biomedical research due to their well-characterized genetic and physiological characteristics.
Automatically generated - may contain errors
Lab products found in correlation
Td 06414, by Inotiv (1 mentions)
D12492, by Research Diets (1 mentions)
Pwk mice, by RIKEN BioResource Center (1 mentions)
Mrl mpj faslpr j, by Jackson ImmunoResearch (1 mentions)
R m h diet, by Ssniff (1 mentions)
1324 standard diet, by Altromin (1 mentions)
Ggpp g6025, by Merck Group (1 mentions)
Ot 1 mice, by Jackson ImmunoResearch (1 mentions)
18 protocols using c57bl 6j b6 mice
1
C57BL/6J Mouse Housing and Care
2
Dietary Manipulations in C57Bl/6J Mice
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Male, C57Bl/6J (B6) mice were purchased at 2 mo old from Charles River Laboratories (Wilmington, MA, USA). After delivery, the mice were allowed to adapt to the animal core facility for 1 wk before the start of the experiment. All mice were group-housed on a 12/12-h dark/light cycle with free access to food and water. If not otherwise stated, the mice received a normal chow diet (R70; Lantmännen, Stockholm, Sweden). The following special diets were fed to 3-mo-old B6 mice for 8 wk: a high-sucrose diet (HSD; 32% sucrose dissolved in tap water); a high-fat diet (HFD; 60% kcal from fat, TD.06414; Envigo, Huntingdon, United Kingdom); a HFHSD (HFD + HSD); a HFHFrD [HFD + high-fructose diet (HFrD; 32% fructose in tap water)]; and a control diet [managed formulation–purified ingredient diet (5P76; LabDiet, St. Louis, MO, USA)]; with tap water. The control diet given to the control group differed from the R70 diet all animals received before and some mice received up to 8 wk after the diet intervention. Consequently, all the animals underwent the same stress provoked by diet change. All experiments were performed in accordance with the Karolinska Institutet’s guidelines for the care and use of animals in research and were approved by the institute’s Animal Ethics Committee.
3
MAVS-KO Mice on B6 Background
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C57BL/6J (B6) mice and MAVS-KO mice were purchased from Charles River Japan (Tokyo) and The Jackson Laboratory (Bar Harbor, ME), respectively. As described previously, MAVS-KO mice were generated and maintained on a B6 background (15 (link)). These mice were all bred and housed at Kanazawa University, where all animal experiments were conducted in accordance with Kanazawa University’s animal care regulations and authorized by the Institute for Experimental Animals at the Kanazawa University Advanced Science Research Center (registration no. AP-194072).
4
Mice Maintenance and Handling Protocol
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C57BL/6J (B6) mice (purchased from Charles River Laboratories France) and Collaborative Cross CC001 and CC071 mice (purchased from the Systems Genetics Core Facility, University of North Carolina and bred at the Institut Pasteur) were maintained under specific-pathogen-free conditions with a 14-h light and 10-h dark cycle and ad libitum food and water in the Institut Pasteur animal facility. In all experiments, mice were killed by cervical dislocation. All experimental protocols were approved by the Institut Pasteur Ethics Committee (dap190107) and authorized by the French Ministry of Research (#19469), in compliance with French and European regulations.
5
High-Fat Diet Feeding in B6 Mice
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Male C57BL6/j (B6) mice were purchased from Charles River Laboratories at the age of 6 weeks. Mice, housed three to five animals per cage, were acclimated to our animal facilities for 2 weeks from arrival in a temperature- and humidity-controlled room with 12-hour light/12-hour dark cycles and ad libitum access to water and food (R70, Lantmännen, Sweden). At the age of 8 weeks, all mice were fed an HFD (60% kcal from fat, Open Source Diets D12492, Research Diets). The duration of the diet intervention varied depending on the experimental design, from 14 to 24 weeks. Animal care and experiments were carried out according to the Animal Experiment Ethics Committee at Karolinska Institutet.
6
Genetic Mapping of Mouse Strains
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C57BL/6J (B6) mice were purchased from Charles River Laboratories France. Collaborative Cross strains (CC001/Unc, CC071/TauUnc, CC005/TauUnc, CC011/Unc, CC026/GeniUnc, CC061/GeniUnc, CC021/Unc, CC006/TauUnc, CC025/GeniUnc, CC039/Unc, CC060/Unc) were purchased from the Systems Genetics Core Facility, University of North Carolina and bred at the Institut Pasteur. Irf3 Irf7 double KO mice (C57BL/6J-Bcl2l12/Irf3tm1TtgIrf7tm1Ttg, [32 (link),42 (link)]) were bred at the Institut Pasteur and backcrossed to B6 mice to generate Irf3 single KO mice (B6-Irf3 KO). Genetic mapping was performed on MEFs derived from (CC001 x CC071) x CC071 and CC071 x (CC001 x CC071) backcross embryos. For the quantitative complementation test, MEFs were derived from CC071, B6-Irf3 KO and (B6-Irf3+/KO x CC071) embryos. In vivo experiments were performed on B6, B6-Irf3 KO, CC071, (CC071 x Irf3 KO) F1 and CC071 x (CC071 x Irf3 KO) backcross mice. All crosses are described as female x male. All mice were maintained as described previously [15 (link)].
7
Dietary impact on B6 and PWK mice
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C57BL/6J (B6) mice were purchased from Charles River Japan. PWK mice were purchased from RIKEN BioResource Center, Tsukuba, Japan. Mice were housed in box cages and maintained on a 12-h light/12-h dark cycle. B6 and PWK male mice and F1 male progeny obtained by reciprocal crosses between them were placed in single cages and fed either a normal diet (Research Diets, New Brunswick, NJ, USA, catalog [cat.] #D06072701) or a HFD (Research Diets, #D07012601) for 15 weeks, starting at the age of 6 weeks.
8
Obesity Mouse Model Characterization
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The ob/ob and ob-control mice, which have a C57BL/6J background, originated from Umeå, Sweden, and were inbred in the animal core facility at Karolinska Hospital. Discrimination between ob/ob and ob-control littermates was achieved by genotypic analysis21 (link). C57BL/6J (B6) mice were purchased from Charles River (Wilmington, MA, USA). All experiments were performed in accordance with the Karolinska Institutet’s guidelines for the care and use of animals in research and were approved by the institute’s Animal Ethics Committee.
9
Lupus-prone Mouse Spleen Analysis
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Genetically lupus-prone female MRL/MpJ-Faslpr/J (MRL) mice, B6.MRL-Faslpr/J mice (B6MRL) (Jackson Laboratory), and C57BL/6 J (B6) mice (Charles River Laboratories) were purchased. At 16 weeks of age, MRL and B6 mice were sacrificed, and their spleen tissues were collected.
10
Dietary Intervention in Normoglycemic Mice
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Male C57BL6/j (B6) mice were purchased from Charles River at the age of six weeks. Mice, housed 5 animals per cage, were acclimated to our animal facilities for two weeks from arrival in a temperature- and humidity-controlled room with 12 h light/dark cycles and ad libitum access to water and food (R70, Lantmännen, Stockholm, Sweden). At the age of eight weeks, normoglycemic mice with similar body weight were assigned to be either fed a high-fat diet (HFD; 60% kcal from fat, Open Source Diets D12492, Research Diets, New Brunswick, NJ, USA) or kept on standard chow diet (R70, Lantmännen, Sweden). The duration of the diet intervention varied depending on the experimental design, from 14 to 24 weeks. Animal care and experiments were carried out according to the Animal Experiment Ethics Committee at Karolinska Institutet (Ethical permit number 19462/2017, date of approval, 08-February-2018).
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