The TAC Assay Kit (BioVision, Milpitas, USA) was used according to the manufacturer’s instructions. Absorbance was measured at 570 nm using a plate reader (iMarkTm Microplate Absorbance Reader, Bio-Rad, Munich, Germany).
Tac assay kit
Manufactured by Abcam
Sourced in United States
The TAC Assay Kit is a laboratory product designed to measure the total antioxidant capacity (TAC) of a sample. The kit provides the necessary reagents and components to perform this analysis.
Automatically generated - may contain errors
Lab products found in correlation
Imark microplate absorbance reader, by Bio-Rad (1 mentions)
Bio plex, by Bio-Rad (1 mentions)
Nitrotyrosine elisa kit, by Abcam (1 mentions)
Lipid hydroperoxide assay kit, by Cayman Chemical (1 mentions)
Total nitric oxide assay kit, by Thermo Fisher Scientific (1 mentions)
8 ohdg elisa kit, by Abcam (1 mentions)
Adi 907 030a, by Enzo Life Sciences (1 mentions)
8 isoprostane eliza kit, by Enzo Life Sciences (1 mentions)
Eliza kit, by Enzo Life Sciences (1 mentions)
8 protocols using tac assay kit
1
Quantification of Cellular Metabolic Activity
2
Inflammatory and Oxidative Stress Profiles in TNFi Therapy
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The inflammatory profile was analyzed in the serum of HD and RA patients both, before and after 6 months of TNFi therapy, by using a multiplex-type immunoassay—Bioplex (Bio-Rad, CA, USA)—in which a panel of 27 cytokines was evaluated.
Oxidative stress parameters were determined through the evaluation of oxidation of both lipids and proteins, along with the analysis of the total antioxidant capacity. Assays of lipid peroxidation levels were carried out using the Thiobarbituric acid reactive substances (TBARS) assay kit (Canvax Biotech, Córdoba, Spain), following the manufacturer's recommendations.
Protein nitrosylation was measured by using the Nitrotyrosine ELISA kit (Abcam, Cambridge, UK), following the manufacturer's recommendations. Serum total antioxidant capacity (TAC) was measured by quantitative colorimetric determination, using TAC Assay kit (Biovision, Mountain View, CA, USA) following the instructions provided by the manufacturer.
Oxidative stress parameters were determined through the evaluation of oxidation of both lipids and proteins, along with the analysis of the total antioxidant capacity. Assays of lipid peroxidation levels were carried out using the Thiobarbituric acid reactive substances (TBARS) assay kit (Canvax Biotech, Córdoba, Spain), following the manufacturer's recommendations.
Protein nitrosylation was measured by using the Nitrotyrosine ELISA kit (Abcam, Cambridge, UK), following the manufacturer's recommendations. Serum total antioxidant capacity (TAC) was measured by quantitative colorimetric determination, using TAC Assay kit (Biovision, Mountain View, CA, USA) following the instructions provided by the manufacturer.
3
Quantifying Cellular Antioxidant Capacity
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The cellular total antioxidant capacity (TAC) was quantified using a TAC assay kit (Biovision, Mountain View, CA), according to the manufacturer’s instructions. The concentration of the TAC was calculated from the standard curves, and the value was expressed as nmol/ng protein.
4
Plasma Antioxidant and Oxidative Biomarkers
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The nitric oxide (NO) stable end products nitrite plus nitrate were measured in plasma using a Total Nitric Oxide Assay kit (Thermo Scientific, Rockford, IL, USA). Serum total antioxidant capacity (TAC) was determined by quantitative colorimetric determination, using a TAC Assay Kit (BioVision, Mountain View, CA, USA). The plasma lipoperoxydes (LPO) were quantified using a Lipid Hydroperoxide assay kit (Cayman Chemical, Ann Arbor, MI, USA).
5
Antioxidant Activity Evaluation in Hepatic Tissue
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TAC was evaluated in serum in line with the method of Koracevic et al. [48 (link)] following the manufacturer’s instructions for the TAC assay kit (Abcam, Cambridge, MA, USA). The activity of SOD and content of MDA were assessed in (20% w/v) hepatic tissue homogenates according to previously described methods [49 (link),50 (link)], respectively. Catalase activity was measured according to the instructions of the commercial kit [51 (link)] (Biodiagnostic, Giza, Egypt).
6
Oxidized LDL and Antioxidant Capacity Assay
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Oxidized low-density lipoprotein (LDL; cat# 10-1143-01) and glutathione peroxidase 1 (GPX-1; cat# ab193767) were measured by an ELISA kit from Mercodia AB (Uppsala, Sweden) and Abcam (USA), respectively. Total antioxidant capacity (TAC) was measured by the TAC Assay Kit (cat# ab65329; Abcam) according to the manufacturer’s protocol. The detection range for oxidized LDL was 1.4–21.3 mU/L and for GPX-1 0.4–25 ng/mL. The intra-assay and interassay variations were 4.2% and 9.8%, respectively.
7
Quantification of Urinary Oxidative Stress Biomarkers
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The quantifications of 8-OHdG, 8-isoprostane, and total antioxidant capacity (TAC) in urine samples were performed in accordance with the manufacturer’s instructions (8-OHdG ELISA kit, Biovision, Waltham, MA, USA, K4160-100; 8-isoprostane ELIZA kit, Enzo, Farmingdale, NY, USA, DI-900-010; and TAC Assay Kit, Abcam, Cambridge, MA, USA, ab52635). The procedures used in the urine biomarker assay were in accordance with our previous report [25 (link)]. The measurements of urine oxidative stress biomarker levels were further standardized based on urinary creatinine levels measured using a commercial kit (Enzo Life Sciences Inc., Farmingdale, NY, USA, ADI-907-030A).
8
Urinary Oxidative Stress Biomarkers Quantification
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The targeted urinary oxidative stress biomarkers included total antioxidant capacity (TAC), 8-isoprostane, and 8-hydroxy-2-deoxyguanosine (8-OHdG). The quantification of 8-OHdG, 8-isoprostane, and TAC in the urine samples was performed in accordance with the respective manufacturer’s instructions (8-OHdG: ELISA kit, BioVision, Boston, MA, USA; 8-isoprostane: ELIZA kit, Enzo, NY, USA; TAC Assay Kit, Abcam, Boston, MA, USA). The laboratory processes were similar to those previously reported [14 (link)].
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