GelNB-loaded molecular weight 3500 Da dialysis bags with deionized water were sealed by clips and immersed in 500 mL of d -Glucose (Meilunbio, China), l -arginine (Sangon Biotech, China), l -glutamic acid (Macklin, China), l -tryptophan (Macklin), glycine (Macklin), l -glutamine (Macklin), l -cysteine (Macklin), or Ringer's solution, respectively. As a control, deionized water was placed in a separate clean dialysis bag. The liquid in the dialysis bags was collected in a vacuum tube at regular intervals as a dialysis test sample. The amino acid concentrations were determined using a total amino acid assay kit (Nanjing Jiancheng Bio-engineering Institute, China). The glucose concentration was assessed using a glucose colorimetric assay kit (Elabscience, China). The ion concentrations were measured using an automatic biochemical analyzer (Beckman Coulter AU5800, USA).
Glucose colorimetric assay kit
Manufactured by Elabscience
Sourced in China
The Glucose Colorimetric Assay Kit is a laboratory equipment designed to quantitatively measure glucose levels in various biological samples. It utilizes a colorimetric reaction to determine the concentration of glucose present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Glycine, by Maclin Biochemical Technology (1 mentions)
L cysteine, by Maclin Biochemical Technology (1 mentions)
L glutamic acid, by Maclin Biochemical Technology (1 mentions)
L glutamine, by Maclin Biochemical Technology (1 mentions)
L tryptophan, by Maclin Biochemical Technology (1 mentions)
Total amino acid assay kit, by Nanjing Jiancheng (1 mentions)
Au5800, by Beckman Coulter (1 mentions)
L arginine, by Sangon (1 mentions)
D glucose, by Meilun (1 mentions)
Lactate colorimetric assay kit, by Abcam (1 mentions)
3 protocols using glucose colorimetric assay kit
1
Dialysis Characterization of Biomolecules
2
Fatty Acid and Glucose Uptake Assay
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Cells were prepared in 12-well plate with 80% confluence in each well. Then cells were treated with 50 μM SSO for 30 min or left untreated. Then supernatant was removed and cells were washed with warmed PBS for two times. For fatty acid assay, 350 μl DMEM with 0.25 mM PA (no serum) was added to each well and the plate was incubated at 37 °C for 40 min. The supernatant was used for FA measurement according to the instructions of the kit (NEFA LabAssay Kit, Cat# 294-63601, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). For glucose uptake assay, 350 μl DMEM with 1 mM glucose (no serum) was added to each well and the plate was incubated at 37 °C for 40 min. The supernatant was used for glucose measurement according to the instructions of the kit (GlucoseColorimetric Assay Kit, Cat# E-BC-K234-S, Elabscience Biotechnology Co., Ltd., Wuhan, China). The cells in each well were lysised with RIPA for protein assay. Finally, the absorbance value of fatty acid or glucose was corrected by the amount of protein in each well.
3
Glucose and Lactate Quantification
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The levels of glucose and lactate in samples were measured using the glucose colorimetric assay kit (Elabscience, Wuhan, China) and lactate colorimetric assay kit (BioVision, Milpitas, California, USA) according to the instructions, respectively. Briefly, the medium from each treatment group were collected and the levels of glucose and lactate in the medium were marked as controls. Next, the cells were incubated for 14 to 18 hours and then the levels of glucose and lactate in the culture medium was measured.
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