Indiplon

All experimental procedures were approved by the Rosalind Franklin University IACUC in accordance with the USPHS Guide for Care and Use of Laboratory Animals. Male Sprague-Dawley rats (Harlan, IN) were allowed to acclimate for at least 5 days before receiving any experimental manipulation. They were group housed (2–3 rats/cage) in a 12:12 hour light/dark cycle room with food and water available ad libitum at 21–23°C. All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) except for WIN, Indiplon, and AM251, which were obtained from Tocris Bioscience (Ellisville, MO).

Cntnap4 knock-in mice used in this study were generated by introducing eGFP in frame with Cntnap4 start codon. To characterize gene expression, eGFP was used to represent Cntnap4-expressing cells in the brain. Western blot analysis for Cntnap4 localization was performed on adult mouse cortex using a Cntnap4 rabbit polyclonal antibody described previously⁸ . Cntnap4-Fc fusion protein was applied to live neuronal cultures for in vitro localization studies. Evoked extracellular dopamine release was measured using fast-scan cyclic voltammetry of P60 in vitro slice preparations in WT, HET and KO animals¹¹ . For paired cell recordings between FS and excitatory neurons, Cntnap4 mice were crossed to a parvalbumin-cre:RFP reporter background to enable targeted physiological recordings. For synaptic ultrastructural analysis, P60 Cntnap4 WT, HET and KO animals were analyzed by electron microscopy, focusing on perisomatic inhibitory synaptic contacts. Cntnap4 mice were assessed by a number of behavioral analyses including grooming score and pre-pulse inhibition^{28 ,29} (San Diego Instruments). Pharmacological rescue was performed using indiplon (Tocris), which was administered acutely by oral gavage as well as haloperidol (Innovative Research of America), which was delivered chronically via subcutaneous slow-release pellet²⁷ .