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Sodium dodecyl sulfate (sds)

Manufactured by Mallinckrodt

Sodium dodecyl sulfate (SDS) is a commonly used anionic detergent in laboratory settings. Its primary function is to denature proteins by disrupting their non-covalent interactions, thereby allowing for their separation and analysis. SDS is a key component in various biochemical techniques, such as gel electrophoresis and Western blotting.

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2 protocols using sodium dodecyl sulfate (sds)

1

RNA Extraction and Purification Protocol

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Tris base, ethylenediaminetetraacetic acid (EDTA), potassium acetate (KoAc), sodium acetate (NaOAc) and lysozyme were purchased from Sigma-Aldrich. TAE running buffer (50×) was purchased from Omega Bio-Tek. Sodium hydroxide (NaOH) was obtained from EM Science. Boric acid was purchased from JT Baker. Sodium dodecyl sulfate (SDS) was purchased from Mallinckrodt Baker. RNase A/T1 Cocktail Mix and RNase V1 were purchased from Invitrogen/Life Technologies. RNase If, 2-log DNA ladder, and dsRNA ladder were purchased from New England Biolabs. RNase A was obtained from Thermo Scientific.
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2

Synthesis and Semihydrogenation of Alkynes

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Anhydrous copper(ii) chloride (CuCl2; 97%) and hydroxylamine hydrochloride (NH2OH·HCl; 99%) were purchased from Aldrich. Sodium hydroxide (98.2%) and sodium dodecyl sulfate (SDS; 100%) were acquired from Mallinckrodt. Diphenylacetylene (DPA; 98%), ammonia borane (H3N–BH3; 97%), anhydrous ethanol (99.5%), ethanol-d (C2H5OD; 99 atom% D), Cu2O (99.99%), and Lindlar catalyst (5% palladium deposited on calcium carbonate poisoned with lead) were purchased from Aldrich. All chemicals were used as received without further purification. Ultrapure distilled and deionized water (18.3 MΩ) was used for all solution preparations. Commercially available reagents were used for the semihydrogenation reactions.
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