IL4, MCSF, IL-6, HGF, IL-10, IL-13, TNF-αR were purchased from Peprotech (Rocky Hill, NJ), GMCSF, TGFb-1 were purchased from R&D (Minneapolis, MN) and IGF-1 from Lonza (Basel, Switzerland). GSK3 inhibitors, Lithium chloride and SB415286, CREB inhibitors, pamoic acid and RO-31-8220, COX2 inhibitor Indomethacin and LPS were purchased from Sigma-Aldrich (St. Louis, MO). EP receptor antagonists, L161982 and Ah6809, and PGE2 were purchased from Cayman Chemical (Ann Arbor, MI). Rabbit polyclonal Abs to CD206 and α-Tubulin were purchased from Abcam (Cambridge, MA). Rabbit monoclonal Abs to phosphorylated CREB (ser-133), GSK-α (ser-21), GSK3-β (ser-9), and IL-6 (tyr-641) as well as to CREB, GSK3-α, GSK3-β, and IL-6 were purchased from Cell Signaling (Boston, MA).
Pamoic acid
Manufactured by Merck Group
Sourced in United States
Pamoic acid is a chemical compound commonly used in laboratory settings. It is a dicarboxylic acid with a molecular formula of C₂₂H₁₆O₆. Pamoic acid serves as a useful tool for various analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sodium citrate na3 citrate dihydrate, by Merck Group (3 mentions)
Millipore wr600a, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Hydrogen tetrachloroaurate trihydrate, by Merck Group (3 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Ro31 8220, by Merck Group (1 mentions)
Gsk3α, by Cell Signaling Technology (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
α tubulin, by Abcam (1 mentions)
Sb415286, by Merck Group (1 mentions)
Tgfb1, by R&D Systems (1 mentions)
Il 13, by Thermo Fisher Scientific (1 mentions)
Ah6809, by Cayman Chemical (1 mentions)
Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
Il 10, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions)
L 161 982, by Cayman Chemical (1 mentions)
9 protocols using pamoic acid
1
Inflammatory Signaling Pathways Modulation
2
Synthesis of Gold Nanoparticles
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EXAMPLE 1
Chemicals
Hydrogen tetrachloroaurate trihydrate (HAuCl4.3H2O), pamoic acid (PA), sodium hydroxide and sodium citrate (Na3-citrate) dihydrate were all purchased from Sigma-Aldrich Company and utilized directly without further purification. All solutions were prepared with ultrapure deionized water obtained from a water purification system (Millipore WR600A, Yamato Co., Japan and/or Barnstead Nanopure, Thermoscientific 7148, USA).
3
Synthesis of PEG-based hydrogel
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PEG 6000 and PEG 1000 were purchased from the USB Corporation, Cleveland, OH, USA. Mg(NO3)2·6H2O and ethyl alcohol, Pamoic acid, and NH3 were purchased from Sigma-Aldrich, St. Louis, MO, USA.
4
Inhibiting DNA Repair Pathways
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Oxaliplatin and cisplatin (Sigma-Aldrich) were dissolved in PBS, aliquoted and stored at -20°C. Treatment concentration and duration are indicated for each experiment. 8-methoxypsoralen (8-MOP, Sigma-Aldrich) dissolved in DMSO was used in 50 μM concentration for 2 h. To inhibit PARP, cells were treated for 24 h with 10 μM olaparib dissolved in PBS (Selleckchem). To inhibit POLB, cells were treated for 24 h with 500 μM pamoic acid (Sigma-Aldrich) dissolved in 200 mM NaCl and 20 mM Tris–HCl. To inhibit transcription, cells were treated for 1 h with 1 μM flavopiridol (Sigma-Aldrich), dissolved in DMSO. Trolox (Sigma-Aldrich) was dissolved in ethanol and applied for 24 h prior to an experiment at a concentration of 600 μM.
5
Pamoic Acid and Zaprinast Regulate Cell Migration
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Cells were seeded in 6-well plates at 3 × 105 cells/well, grown to confluence and then quiesced for 48 or 24 h for VSMC and EC, respectively. Three horizontal scratches per well were created before stimulation with various concentrations of pamoic acid (Sigma-Aldrich) or zaprinast in serum-free DMEM. In a number of experiments, a GPR35 agonist was coadded with 100 nM of either of the human-specific GPR35 antagonists, CID-2745687 and ML-145. In equivalent studies, the structurally and mechanistically distinct Rho A pathway inhibitors, Y-27632 [30 (link),31 (link),32 (link)] or Y-16 [33 (link)], were coadministered with a GPR35 agonist. Images of the cells were acquired at time zero, followed by incubation at 37°C. The cells were imaged up to a maximum period of 36 h. Percentage migration was assessed by measuring the distance (μm) between cells migrating into the wound area in 10 random fields of view per scratch over the time course using ImageJ software.
6
Synthesis of Gold Nanoparticles
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Example 1
Chemicals
Hydrogen tetrachloroaurate trihydrate (HAuCl4.3H2O), pamoic acid (PA), sodium hydroxide and sodium citrate (Na3-citrate) dihydrate were all purchased from Sigma-Aldrich Company and utilized directly without further purification. All solutions were prepared with ultrapure deionized water obtained from a water purification system (Millipore WR600A, Yamato Co., Japan and/or Barnstead Nanopure, Thermoscientific 7148, USA).
7
Recombinant HMGB1 and CXCL12 Production
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Recombinant HMGB1 constructs (Accession code P63158, residues 1–215 and BoxA, residues 1–89) and recombinant human CXCL12 in labeled and unlabeled forms were produced as described (De Leo et al. 2019 (link)). Proteins used for cell-based assays were provided by HMGBiotech (Milan). Pamoic acid was purchased from Sigma Aldrich.
8
Biochemical Assay Materials Compendium
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Imetit, histamine, poly-D-lysine, isoprenaline, and U-46619 were obtained from Sigma Aldrich (Merck KGaA). Zaprinast, kynurenic acid, and pamoic acid were from Sigma-Aldrich, Tocris Bioscience and Sigma-Aldrich, respectively. The Nluc substrate furimazine and the HaloTag fluorescent ligand NanoBRET 618 were from Promega (Madison). Coelenterazine 400a was from Biosynth. White-wall, white-bottomed 96-well, black-wall, black bottomed 96-well, as well as flat-bottomed transparent microtiter plates were from Gibco.
9
Synthesis of Gold Nanoparticles
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Example 1
Chemicals
Hydrogen tetrachloroaurate trihydrate (HAuCl4.3H2O), pamoic acid (PA), sodium hydroxide and sodium citrate (Na3-citrate) dihydrate were all purchased from Sigma-Aldrich
Company and utilized directly without further purification. All solutions were prepared with ultrapure deionized water obtained from a water purification system (Millipore WR600A, Yamato Co., Japan and/or Barnstead Nanopure, Thermoscientific 7148, USA).
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