The following antibodies were used: anti‐TMX1 (SIGMA, HPA003085), anti‐BiP (BD Biosciences, San Jose, CA, USA, 610978), anti‐α‐tubulin (Wako, 017‐25031), anti‐PDI (Enzo Life Sciences, Farmingdale, NY, USA, SPA‐890), anti‐P5 (Thermo Fischer Scientific, PA3‐008), anti‐phospho‐eIF2α (Cell Signaling Technology, Danvers, MA, USA, 9721), and anti‐eIF2α (Cell Signaling Technology, 9722).
Anti α tubulin
Manufactured by Fujifilm
Sourced in United States, Japan
Anti-α-tubulin is a primary antibody used in laboratory research. It specifically binds to the α-tubulin subunit of the microtubule cytoskeleton, allowing for the detection and visualization of microtubules within cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho eif2α, by Cell Signaling Technology (1 mentions)
Anti p5, by Thermo Fisher Scientific (1 mentions)
Anti pdi, by Enzo Life Sciences (1 mentions)
Anti bip, by BD (1 mentions)
Anti eif2α, by Cell Signaling Technology (1 mentions)
Anti c myc 9e10, by Santa Cruz Biotechnology (1 mentions)
Las 4000 system, by Fujifilm (1 mentions)
Anti vdac1, by Abcam (1 mentions)
Anti synaptotagmin, by BD (1 mentions)
Anti aβ antibody 82e1, by Tecan (1 mentions)
Anti flotillin 1, by BD (1 mentions)
Anti glur1, by Merck Group (1 mentions)
Anti nr2b, by BD (1 mentions)
Anti cox 4, by Cell Signaling Technology (1 mentions)
4 protocols using anti α tubulin
1
Immunoblotting of ER Stress Markers
2
Thapsigargin-Induced ER Stress Analysis
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Cells were pretreated with the indicated concentration of GSK2656157 for 1.5 h, followed by the addition of 100 nM thapsigargin for the indicated times. Cells were washed with PBS containing 50 mM NaF, 17.5 mM β-glycerophosphate, and 100 µM Na3VO4 and lysed with LysisB (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% sodium dodecyl sulfate (SDS), 100 mM NaF, 17.5 mM β-glycerophosphate, 100 µM Na3VO4, 10% glycerol). Cleared lysates were mixed with Laemmli sample buffer containing 0.6 M β-mercaptoethanol, followed by boiling, SDS–PAGE, and immunoblotting. Antibodies for immunoblotting were as follows: anti-CREB-2 (ATF4, C-20, Santa Cruz, Dallas, TX), anti-c-Myc (9E10, Santa Cruz), and anti-α-tubulin (Wako, Osaka, Japan).
3
Protein Extraction and Western Blot Analysis
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Dissected cerebral cortices and hippocampi were homogenized in lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.1% H2O2, and 5 mM Na3VO4). Insoluble debris was removed by centrifugation (10 min, 17,800 g), and supernatants were diluted with 4 x SDS-PAGE loading buffer (0.25 M Tris-HCl, pH 6.8, 40% (w/v) glycerol, 8% SDS, 20% (v/v) 2-ME, 0.2% BPB). Samples were separated by SDS-PAGE (5% and 8% separating gels for Reelin and Dab1, respectively). Western blotting was performed as described previously22 (link). Anti-Reelin G10 (1:1,000) antibody was purchased from Millipore. Anti-Dab1 monoclonal antibodies (1:1,000) were described previously60 (link). Anti-α-tubulin (1:10,000) was purchased from Wako Pure Chemicals (Osaka, Japan). Images were captured using a LAS4000 system (Fuji, Tokyo, Japan) and were quantified with ImageJ software (version 1.48v, National Institutes of Health, MD) as described previously23 (link).
4
Antibody Sourcing for Neurodegenerative Research
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Anti-synaptotagmin, anti-PSD95, and anti-flotillin-1 antibodies were purchased from BD Bioscience (Franklin Lakes, NJ, USA). Anti-actin (Chemicon/Millipore, Billerica, MA, USA), anti-COX IV (Cell Signaling, Danvers, MA, USA), anti-α-tubulin (Wako Pure Chemical Industries, Osaka, Japan), and anti-VDAC1 (Abcam, Cambridge, UK) antibodies were purchased from the indicated suppliers. Anti-APP cytoplasmic antibody (G369, from Dr. Gandy) to detect APP FL and CTF, 13 anti-BACE1 (3D5, from Dr.Vassar), 14 and anti-PS1 CTF (G1L3, from Dr. Tomita) 15 were obtained as gifts from the indicated sources. Mouse monoclonal anti-Aβ antibodies 6E10 (raised against human Aβ1-16) and 4G8 (raised against human Aβ17-24) were purchased from BioLegend (San Diego, CA, USA), and anti-Aβ antibody 82E1 (raised against human Aβ1-16) was purchased from IBL (Fujioka, Gunma, Japan). Anti-GluR1 (Merck Millipore, Darmstadt, Germany), anti-NR2A (Thermo Fisher Scientific, Waltham, MA, USA), and anti-NR2B (BD Biosciences) antibodies were purchased from the indicated suppliers.
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