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Chenmidoc xrs

Manufactured by Bio-Rad

The Bio-Rad ChemiDoc™ XRS+ is a compact and versatile imaging system designed for a range of molecular biology applications. It captures high-quality images of various sample types, including gels, blots, and microplates, using a sensitive charge-coupled device (CCD) camera.

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2 protocols using chenmidoc xrs

1

Protein Expression Analysis in Skin Tumors

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Western blotting assay was performed as follows: the protein samples were collected from the human skin tumor or cells. The tissue lysates were separated on 12% SDS-polyacrylamide gels and transferred onto polyvinylidene fluoride membranes (Bio-Rad). Antibodies used included TERT antibody (1:1000), snail antibody (1:1000), E-cadherin antibody (1:1000), while GAPDH (1:2000) was used as a loading control. The Western blot results were further analyzed using BIO-RAD ChenmiDoc™ XRS+.
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2

Protein Expression Analysis by Western Blot

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Western blotting was performed as follows: the protein lysates were collected from the cells. The tissue lysates were separated on 12% SDS-polyacrylamide gels and transferred onto polyvinylidene fluoride membranes (Bio-Rad). Antibodies used included CK10, CK1 (1:1000, Abcam), Involucrin, IL-6, p-ERK and ERK antibody (1:1000, Cell Signaling Technology)), while GAPDH (1:2000) was used as a loading control. The Western blot results were further analyzed using BIO-RAD ChenmiDoc™ XRS+.
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