Alkaline phosphatase conjugated goat anti rabbit or anti mouse immunoglobulin g secondary antibodies

Manufactured by Jackson ImmunoResearch

Sourced in United States

Alkaline phosphatase conjugated goat anti-rabbit or anti-mouse immunoglobulin G secondary antibodies are laboratory reagents used to detect and quantify the presence of target proteins in biological samples. These antibodies are designed to bind to the Fc region of rabbit or mouse primary antibodies, and the conjugated alkaline phosphatase enzyme provides a means to visualize and measure the target proteins.

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Lab products found in correlation

Anti p24 mouse mab, by Merck Group (2 mentions) Anti ha mouse monoclonal antibody mab, by Fortrea (2 mentions) Anti tubulin mouse mab, by Fortrea (2 mentions) Anti his mouse mab, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti flag mab, by Merck Group (1 mentions) Anti gapdh rabbit polyclonal antibody, by Affinity Biosciences (1 mentions)

2 protocols using alkaline phosphatase conjugated goat anti rabbit or anti mouse immunoglobulin g secondary antibodies

Antibody Usage in Biological Research

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The following antibodies were used in this study: anti-HA mouse monoclonal antibody (mAb) (Covance, Emeryville, CA, USA), anti-tubulin mouse mAb (Covance), anti-His mouse mAb (Sigma, St. Louis, MO, USA) and anti-p24 mouse mAb obtained from an HIV-1 p24 hybridoma from the National Institutes of Health AIDS Research and Reference Reagent Program (NIH-ARRRP).
Alkaline phosphatase conjugated goat anti-rabbit or anti-mouse immunoglobulin G secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Alexa Fluor 488 goat anti-mouse IgG secondary antibodies and Alexa Fluor 633 goat anti-rabbit IgG secondary antibodies were from Invitrogen.

Wang W., Wang J., Qu M., Li X., Zhang J., Zhang H., Wu J., Yu B., Wu H., Kong W, & Yu X. (2015). Viral Restriction Activity of Feline BST2 Is Independent of Its N-Glycosylation and Induction of NF-κB Activation. PLoS ONE, 10(9), e0138190.

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Antibody Characterization and Detection

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The following antibodies were used: anti-HA mouse monoclonal antibody (mAb) (Covance), anti-tubulin mouse mAb (Covance), anti-NF-κB 1 mouse mAb (Affinity Bioscience), anti-NF-κB p65 rabbit polyclonal antibody (Affinity Bioscience), anti-GAPDH rabbit polyclonal antibody (Affinity Bioscience), anti-Histone H3 rabbit polyclonal antibody (Proteintech), anti-Flag mouse mAb (Sigma), and anti-p24 mouse mAb obtained from an HIV-1 p24 hybridoma from the National Institutes of Health AIDS Research and Reference Reagent Program. Alkaline phosphatase-conjugated goat anti-rabbit or anti-mouse immunoglobulin G secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove).

Qu M., Wang W., Li W., Cao J., Zhang X., Wang C., Wu J., Yu B., Zhang H., Wu H., Kong W, & Yu X. (2020). Antiviral Activity of Feline BCA2 Is Mainly Dependent on Its Interference With Proviral Transcription Rather Than Degradation of FIV Gag. Frontiers in Microbiology, 11, 1230.

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