Milliplex rat kits

Manufactured by Merck Group

Sourced in Spain

The Milliplex Rat kits are a range of multiplex assay kits developed by Merck Group for the quantitative measurement of various analytes in rat biological samples. These kits utilize Luminex xMAP technology, which allows for the simultaneous detection and quantification of multiple analytes from a single sample. The core function of the Milliplex Rat kits is to provide researchers with a reliable and efficient tool for analyzing the levels of specific proteins, cytokines, or other biomolecules in rat models.

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Lab products found in correlation

Adiponectin, by Merck Group (2 mentions) Rat leptin elisa kit, by Merck Group (1 mentions) Mcp 1 elisa kit, by RayBiotech (1 mentions) Adiponectin, by Thermo Fisher Scientific (1 mentions) Au5400, by Beckman Coulter (1 mentions) Architect i2000sr, by Abbott (1 mentions)

2 protocols using milliplex rat kits

Multiplex Biomarker Quantification

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Interleukin-1 beta (IL-1β), IL-10), tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta 1 (TGF-β1) were measured with custom Milliplex Rat kits (Merck Millipore, Madrid, Spain), using Luminex xMAP Multiplexing Technology platform. Leptin, adiponectin and monocyte chemoattractant protein-1 (MCP-1) were determined using, respectively, Rat Leptin ELISA Kit (Merck Millipore, Madrid, Spain), Adiponectin Enzyme Immunoassay Kit and MCP-1 ELISA Kit (RayBiotech, Norcross, USA).

Pestana D., Teixeira D., Meireles M., Marques C., Norberto S., Sá C., Fernandes V.C., Correia-Sá L., Faria A., Guardão L., Guimarães J.T., Cooper W.N., Sandovici I., Domingues V.F., Delerue-Matos C., Monteiro R., Constância M, & Calhau C. (2017). Adipose tissue dysfunction as a central mechanism leading to dysmetabolic obesity triggered by chronic exposure to p,p’-DDE. Scientific Reports, 7, 2738.

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Comprehensive Biomarker Quantification Protocol

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Serum glucose, uric acid, C-reactive protein (CRP), LDL, and HDL cholesterol were quantified using conventional methods with an Olympus AU5400 automated clinical chemistry analyzer (Beckman-Coulter, Izasa, Porto, Portugal). Homocysteine was measured by chemiluminescent immunoassay using an Architect i2000 SR automated analyzer (Abbott).
Serum folate was quantified by time-resolved fluoroimmunoassay using an AutoDelfia automated analyzer (PerkinElmer Life and Analytical Sciences, Wallac Oy, Turku, Finland).
Plasma insulin, leptin, and adiponectin were quantified by ELISA using commercially available kits according to the manufacturer's instructions (insulin and leptin: Millipore, St Charles, MO, USA; adiponectin: Invitrogen Corporation). According to the instructions, if the difference between duplicate results of a sample was over O15% (of the coefficient of variation), the sample was repeated whenever it was possible. Sensitivities of the assays were 0.2 ng/ml (insulin), 0.04 ng/ml (leptin), and 50 pg/ml (adiponectin).
Serum interleukin 1 beta (IL1b), interleukin 10 (IL10), and transforming growth factor-beta 1 (TGFb1) were measured by Luminex assay using the custom Milliplex Rat kits (Merck Millipore, Madrid, Spain), according to the manufacturer's protocols, by Luminex xMAP Multiplexing Technology platform.

Keating E., Correia-Branco A., Araújo J.R., Meireles M., Fernandes R., Guardão L., Guimarães J.T., Martel F, & Calhau C. (2015). Excess perigestational folic acid exposure induces metabolic dysfunction in post-natal life. The Journal of endocrinology, 224(3).

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