Mas coat

Manufactured by Matsunami

Sourced in Japan

The MAS COAT is a laboratory coating equipment designed for applying thin, uniform coatings on various substrates. The core function of the MAS COAT is to provide a controlled and consistent coating process, enabling the creation of precise and reproducible coating layers.

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Lab products found in correlation

Alexa fluor 647 donkey anti sheep igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 546 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Non immune horse serum, by Vector Laboratories (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Oct compound, by Sakura Finetek (1 mentions) Bz x710, by Keyence (1 mentions) Fluoview fv10i, by Olympus (1 mentions) Pentobarbital sodium, by Nacalai Tesque (1 mentions) Bz x700 microscope, by Keyence (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions) Fluoromount, by Diagnostic BioSystems (1 mentions)

6 protocols using mas coat

Immunostaining of Ocular Tissues

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For immunostaining the ocular tissues, the eyes were enucleated and fixed in 4% paraformaldehyde for at least 24 h at 4⁰ C, and then immersed in 25% sucrose in 0.01 M phosphate buffered sarin (PBS) for 2 days. The eyes were then embedded in optimal cutting temperature (OCT) compound (Sakura Finetek Japan) and immediately frozen with liquid nitrogen. Ten micrometer sections were cut with a cryostat, and the sections were mounted on glass slides (MAS COAT; Matsunami Glass).
The retinal sections were blocked in non-immune horse serum (Vector Labs) for 1 h, and then incubated with the primary antibody at 4⁰ C overnight. The next morning the sections were covered with a secondary antibody for 1 h and then counterstained with Hoechst 33342 (1:1000; Invitrogen, catalog H3570) for 15 min.
The following antibodies were used; sheep anti-mPGRN (1:100; R&D Systems, catalog AF2557), rabbit anti-Iba1 (1:200; FUJIFILM Wako Chemicals, catalog 019-19741), Alexa Fluor ® 647 donkey anti-sheep IgG (1:1000; Invitrogen, catalog A21448), and Alexa Fluor ® 546 donkey anti-rabbit IgG (1:1000; Invitrogen, catalog A10040). The immunostained sections were examined and photographed with a confocal microscope (FLUOVIEW FV10i; Olympus) or BZ-X710 (Keyence).

Takahashi K., Nakamura S., Otsu W., Shimazawa M., & Hara H. (2021). Progranulin Deficiency in Iba-1+ Myeloid Cells Exacerbates Choroidal Neovascularization by Perturbation of Lysosomal Function and Abnormal Inflammation.

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Perfusion-Fixation and Tissue Sectioning

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Mice were anesthetized with an intraperitoneal injection of 50 mg/kg sodium pentobarbital (Nacalai Tesque, Inc., Kyoto, Japan), perfused with saline and then immediately perfused with 4% paraformaldehyde (PFA) for 8 min. The brain, eye, and optic nerve were removed and further fixed in 4% PFA for 24 h at 4 °C. The tissues were immersed in 25% sucrose solution for 24 h at 4 °C and frozen in optimal cutting temperature compound (Sakura Finetechnical Co., Ltd., Tokyo, Japan). Finally, the brain (12 μm) and eye and optic nerve (10 μm) were sectioned and mounted on glass slides (MAS COAT; Matsunami Glass Ind., Ltd., Osaka, Japan).

Kuse Y., Tsuruma K., Mizoguchi T., Shimazawa M, & Hara H. (2017). Progranulin deficiency causes the retinal ganglion cell loss during development. Scientific Reports, 7, 1679.

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Immunohistochemical Analysis of Skin Sections

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For immunohistochemistry, skin sections of mice placed on slides (MASCOAT, Matsunami, Osaka, Japan) were deparaffinized with immersion in dimethylbenzene, rehydrated, heated in citrated buffer (0.01 M, pH 6.0) for 5 min at 100°C, and then treated with endogenous peroxidase (3% hydrogen peroxide solution) for 5 min at room temperature. After blocking in 10% goat serum for another 1 h at room temperature, the sections were immunostained with primary antibodies for CXCL10, CXCR3, and tyrosinase diluted in 0.01 M PBS containing 0.3% (v/v) Triton X-100 and 5% bovine serum albumin overnight at 4°C. The sections were washed with 0.01 M PBS, incubated with biotinylated anti-rabbit IgG before being incubated with the avidin-biotin-peroxidase complex for 30 min at room temperature, and finally visualized using aminoethyl carbazole (AEC) as a peroxidase substrate. Images were captured under an Olympus BX51 microscope installed with ImageJ software.

Guan C., Li Q., Song X., Xu W., Li L, & Xu A. (2017). Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxidative Medicine and Cellular Longevity, 2017, 9303054.

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Immunofluorescence Analysis of H3S10p

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tsFT210 cells were washed once with PBS (−), cross-linked in 4% paraformaldehyde at room temperature for 10 min. The cells were washed twice in PBS (−) and then fixed on a slide glass (MAS coat; Matsunami Glass Ind., Ltd., Osaka, Japan) by incubated at 37 °C for 40 min. The slides were washed twice with PBS (−), followed by permeabilization with 0.1% Triton X-100 at 4 °C for 5 min. After washes with PBS (−), cells were blocked for 1 h with 5% BSA in PBS (−). Primary antibody (anti-H3S10p [CST, Danvers, MA, USA; #53348, 1:1600]) diluted in 1% BSA in PBS (−) was added and incubated at room temperature for 1 h. After three washes with PBS (−), secondary antibodies (coupled to AlexaFluor 564) and DAPI (Wako Pure Chemical Industries Ltd., Osaka, Japan) diluted in 1% BSA in PBS (−) were added and incubated at room temperature for 1 h. The slides were washed again three times with PBS (−) and mounted with fluorescence mounting medium (Agilent Technologies, Santa Clara, CA, USA). The fluorescence images were obtained using a BZ-X700 microscope (Keyence, Osaka, Japan).

Goto S., Takahashi M., Yasutsune N., Inayama S., Kato D., Fukuoka M., Kashiwaba S.I, & Murakami Y. (2019). Identification of GA-Binding Protein Transcription Factor Alpha Subunit (GABPA) as a Novel Bookmarking Factor. International Journal of Molecular Sciences, 20(5), 1093.

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Tissue Preparation for Retinal Analysis

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Eyes were enucleated at 24 and 48 h after light exposure and fixed in 4% paraformaldehyde (PFA; Wako) in 0.1 M phosphate buffer (pH 7.4; Wako) overnight at 4 °C. Following fixation, the eyes were cryoprotected with 25% sucrose in 0.1 M phosphate buffer (pH 7.4) for 48 h at 4 °C. The eyes were then embedded in optimal cutting temperature (OCT) compound (Sakura Fine Technical Co., Ltd., Tokyo, Japan) and rapidly frozen on dry ice. Sections (10 µm thick) were sliced on a cryostat and placed on glass slides (MAS COAT; Matsunami Glass Ind., Ltd., Osaka, Japan).

Tanaka M., Kuse Y., Nakamura S., Hara H, & Shimazawa M. (2019). Potential effects of progranulin and granulins against retinal photoreceptor cell degeneration. Molecular Vision, 25, 902-911.

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Transcardial Perfusion and Brain Sectioning

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One week following tracer injection, rats were deeply anesthetized with pentobarbital (240 mg/kg body weight, intraperitoneal; Kyoritsu Pharmaceutical, Tokyo, Japan) and transcardially perfused with 200 ml of 0.01 M phosphate-buffered saline, followed by 200 ml of 4% phosphate-buffered paraformaldehyde. After cryoprotection with 10%, 20%, and 30% sucrose, brains were embedded in Tissue-Tec (O.C.T. Compound; Sakura, Tokyo, Japan) and rapidly frozen in a dry ice/ethanol bath. Brain and spinal cord tissues were cut into 20 μm thick coronal sections on a cryostat. Brain sections were prepared in 500 μm intervals from the rostral-most tip to the caudal end of medulla oblongata. The spinal cord sections were prepared at the C7 and C8 levels for injection validation. Frozen sections were mounted on hydrophilic glass (MAS coat; Matsunami, Osaka, Japan) and coverslipped with Fluoromount (Diagnostic BioSystems, Pleasanton, CA, USA).

Okabe N., Himi N., Maruyama-Nakamura E., Hayashi N., Narita K, & Miyamoto O. (2017). Rehabilitative skilled forelimb training enhances axonal remodeling in the corticospinal pathway but not the brainstem-spinal pathways after photothrombotic stroke in the primary motor cortex. PLoS ONE, 12(11), e0187413.

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