U343-Luc and U251X7-Luc glioma-bearing mice were injected i.v. with coelenterazine (15 μg/mouse). 5 min later, mice were anaesthetized and processed for image analysis using a Xenogen IVIS Lumina imaging system (Caliper, PerkinElmer) for 1–5 min. Tumor growth was imaged once a week starting on day 5 post implantation until mice reached a humane endpoint. Imaging data were analyzed and quantified with the Living Image Software for IVIS Lumina II (PerkinElmer).
Xenogen ivis lumina imaging system
Manufactured by PerkinElmer
The Xenogen IVIS Lumina imaging system is a laboratory instrument designed for in vivo bioluminescence and fluorescence imaging. It provides high-sensitivity detection and quantification of light-emitting reporter genes and labeled probes within small living animals or other samples.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using xenogen ivis lumina imaging system
1
In Vivo Bioluminescence Imaging of Glioma
2
Photothermal and Photodynamic Characterization of SP3NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SP3NPs (100 μg ml-1) in PBS were irradiated for 10 min using a BWF2 continuous-wave NIR laser (808 nm, 0.8 W cm-2; B&W Tek Inc., Newark, DE). Changes in absorbance spectra and morphology of SP3NPs during irradiation were monitored using a UV-Vis spectrophotometer and TEM, respectively. The appearance of SP3NPs during NIR irradiation was recorded using a digital camera.
Fluorescence images of free cypate and SP3NPs in PBS at the same concentrations were recorded with a Xenogen IVIS Lumina imaging system (Perkin Elmer Inc., Waltham, MA) with a built-in ICG filter set using an exposure time of 5 s. The fluorescence intensity in each region of interest (ROI) was semi-quantitated by Xenogen Living Image®, and plotted against the concentration of free cypate and SP3NPs. Photodynamic effects were tested by irradiating free cypate and SP3NPs (25 μg ml-1) in the presence and absence of sodium azide (100 μM) for 5 min (808 nm, 0.8 W cm-2), and the generated singlet oxygen was detected with SOSG following the manufacturer's instructions. PBS served as a negative control. Photothermal effects were assessed by irradiation of different concentrations of SP3NPs with an 808-nm NIR laser (0.8 W cm-2, 5 min). Temperatures were quantified using an IR thermal imaging system (FLIR T420; FLIR Systems Inc., Danderyd, Sweden). The photothermal heating curve of PBS was measured as a negative control.
Fluorescence images of free cypate and SP3NPs in PBS at the same concentrations were recorded with a Xenogen IVIS Lumina imaging system (Perkin Elmer Inc., Waltham, MA) with a built-in ICG filter set using an exposure time of 5 s. The fluorescence intensity in each region of interest (ROI) was semi-quantitated by Xenogen Living Image®, and plotted against the concentration of free cypate and SP3NPs. Photodynamic effects were tested by irradiating free cypate and SP3NPs (25 μg ml-1) in the presence and absence of sodium azide (100 μM) for 5 min (808 nm, 0.8 W cm-2), and the generated singlet oxygen was detected with SOSG following the manufacturer's instructions. PBS served as a negative control. Photothermal effects were assessed by irradiation of different concentrations of SP3NPs with an 808-nm NIR laser (0.8 W cm-2, 5 min). Temperatures were quantified using an IR thermal imaging system (FLIR T420; FLIR Systems Inc., Danderyd, Sweden). The photothermal heating curve of PBS was measured as a negative control.
3
Doxorubicin Uptake in KB-V1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KB-V1 cells were placed on a 35 mm confocal petri dish and treated with Dsi RNA nanoparticles (20 μg/mL) and Doxorubicin (30 μg/mL) in the two-step Dsi-24h-Dox-24h sequential treatment as mentioned above. The cells were washed three times with DPBS 3 h after doxorubicin treatment, and 9 h later, the fluorescence signals, emitted from the intracellular doxorubicin on KB-V1 cells, were examined by a Carl Zeiss LSM710 laser-scanning confocal microscope. To quantitatively measure concentration of the intracellular doxorubicin, we first plated KB-V1 cells on 96-well plates at a density of 2 × 104 cells/well, and then treated the cells with doxorubicin alone or with both RNA nanoparticles and doxorubicin in the aforementioned method. Next, we washed the cells three times using DPBS 3 h after doxorubicin treatment, and 9 h later, measured the fluorescence signals using a Xenogen IVIS Lumina imaging system (Perkin Elmer) and Image J software (NIH).
4
Monitoring siRNA Release from PLGA Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using Cy5.5-labeled siRNA@PLGA NPs, release kinetics of siRNA from PLGA nanoparticles was examined. Cy5.5-siRNA@PLGA NPs were suspended in PBS buffer with gentle stirring, and then a small volume of sample solutions were taken in triplicate at the indicated time points. After the nanoparticles were centrifuged and disintegrated in DMSO, the fluorescence intensity of Cy5.5-siRNA was detected by Xenogen IVIS Lumina imaging system (Perkin Elmer).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!