Anti mouse hrp ecl secondary

Cellular lysates in this study were generally prepared with an NP40-based lysis buffer. The exception is the sample used for the pan-keratin blot, which required a triton-based lysis procedure followed by cytoskeleton protein extraction and urea treatment. Extracted proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and subjected to dry transfer with an iBlot System (Life Technologies, Carlsbad, CA, USA). Antibodies for immunoblotting included the following: anti-β-actin (Millipore, Billerica, MA, USA, product: MAB1501), anti-α6-integrin (Cell Signaling Technology, Boston, MA, USA, product: 3750), anti-pan-cytokeratin (Thermo Scientific, Waltham, MA, USA, product: MA5-13203), anti-slug (Cell Signaling Technology, Boston, MA, USA, product: 3750), anti-tubulin (Cell Signaling Technology, USA, Boston, MA, product: 2148), anti-vimentin (Cell Signaling Technology, Boston, MA, USA, product: 9585), anti-mouse HRP ECL secondary (GE Healthcare, Little Chalfont, UK, product: NA931V), anti-rabbit HRP ECL secondary (GE Healthcare, Little Chalfont, UK, product: NA934V).