Cell cycle was analyzed by propidium iodide (PI) staining as described previously29 (link). In brief, cells were digested, washed with PBS, and fixed in the ice-cold 70% ethanol at −20 °C overnight. Then cells were stained with 40 μg/mL of PI staining buffer (Sigma) at room temperature for 30 min. Following analysis by the Accuri C6/FACSCalibur Flow Cytometer (BD Biosciences, USA), cell cycle data were processed with ModFit LT software (Verity Software House). All experiments were performed in triplicate.
Accuri c6 facscalibur flow cytometer
Manufactured by BD
Sourced in United States
The Accuri C6/FACSCalibur Flow Cytometer is a laboratory instrument designed for the analysis and sorting of cells and particles in suspension. It utilizes laser technology to detect and measure the physical and fluorescent characteristics of individual cells or particles as they flow through the instrument.
Automatically generated - may contain errors
3 protocols using accuri c6 facscalibur flow cytometer
1
Cell Cycle Analysis by PI Staining
2
Apoptosis and Cell Cycle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the apoptosis assay, cells were labeled with an Annexin V-APC/PI Apoptosis Detection Kit (KeyGEN, Nanjing, China) according to the manufacturer’s instructions. For cell cycle analysis, cells were harvested, washed with ice-cold PBS, fixed in 70% ethanol at 4 °C overnight, and labeled with PI (Solarbio, Beijing, China) in the presence of RNase A (Solarbio, Beijing, China) at 37 °C for 30 min. Then, the cells were read on an Accuri C6/FACSCalibur Flow Cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and the data were processed with FlowJo/ModFit LT software.
3
Annexin V-FITC/PI Flow Cytometry Assay for Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The early apoptosis rate was measured using Annexin V-FITC/PI double staining and a Accuri™ C6 FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA) with BD CFlow Software v.264.15 (BD Biosciences). Following treatment with 0, 20 or 40 µM POA for 24 h, 5×105 HK-2 cells were harvested by centrifugation at 800 × g for 5 min at 4°C, washed twice with ice-cold PBS and resuspended in 500 µl binding buffer, followed by the addition of 5 µl Annexin V-FITC conjugate and 5 µl PI buffer, according to the manufacturer's protocol. Following incubation in the dark for 15 min at room temperature, the cells were analyzed by flow cytometry. Each determination is based on the acquisition of 10,000 events (8 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!