Dpni treatment

Manufactured by Thermo Fisher Scientific

DpnI is a restriction enzyme that cleaves double-stranded DNA at the palindromic sequence 5'-Gm6ATC-3'. It is commonly used in molecular biology applications to selectively digest methylated DNA.

Automatically generated - may contain errors

Lab products found in correlation

Accuprime pfx dna polymerase, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

DpnI (132 citations)

2 protocols using dpni treatment

IKAROS Family Zinc Finger Cloning

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human IKAROS family zinc finger 1 (IKAROS) (IKZF1; NM_006060) vector information was previously described (19 ). Human IKAROS family zinc finger 3 (IKZF3/AIOLOS) ORF clone (pcDNA3.1-AIOLOS, NM_012481) and mouse Ikzf1 ORF clone (pcDNA3.1(+)C-HA –Ikzf1, NM_001025597.2) were purchased from GenScript and Ikzf1 was sub-cloned into pFlag-CMV2. Mutants for the IKZF1 were generated based on the site directed mutagenesis protocol using AccuPrime Pfx DNA Polymerase, followed by DpnI treatment (Life technologies).

Boast B., Miosge L.A., Kuehn H.S., Cho V., Athanasopoulos V., McNamara H.A., Sontani Y., Mei Y., Howard D., Sutton H.J., Omari S.A., Yu Z., Nasreen M., Andrews T.D., Cockburn I.A., Goodnow C.C., Rosenzweig S.D, & Enders A. (2021). A point mutation in IKAROS ZF1 causes a B cell deficiency in mice. Journal of immunology (Baltimore, Md. : 1950), 206(7), 1505-1514.

+ Open protocol

+ Expand

Cloning and Mutagenesis of IKZF3 (AIOLOS)

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

IKAROS (GenBank accession no. NM_006060) plasmids used in this study were previously described (Kuehn et al., 2016 (link)). Human AIOLOS (IKZF3) open reading frame clone (pcDNA3.1/C-(K)DYK-IKZF3; NCBI accession no. NM_012481) was purchased from GenScript and subcloned into the mammalian expression vector pcDNA3-HA. Indicated mutants for IKZF3 were generated based on the site-directed mutagenesis protocol using AccuPrime Pfx DNA Polymerase, followed by DpnI treatment (Life Technologies).

Kuehn H.S., Chang J., Yamashita M., Niemela J.E., Zou C., Okuyama K., Harada J., Stoddard J.L., Nunes-Santos C.J., Boast B., Baxter R.M., Hsieh E.W., Garofalo M., Fleisher T.A., Morio T., Taniuchi I., Dutmer C.M, & Rosenzweig S.D. (2021). T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients. The Journal of Experimental Medicine, 218(12), e20211118.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!