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On targetplus short interfering si rnas

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ON-TARGETplus short interfering (si)RNAs are synthetic RNA molecules designed to temporarily silence specific gene expression in cells. They function by binding to and degrading target mRNA, preventing the production of the corresponding protein.

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Lab products found in correlation

Taqman one step pcr master mix, by Thermo Fisher Scientific (2 mentions) Akt sc 5298, by Santa Cruz Biotechnology (1 mentions) β actin antibody a5441, by Merck Group (1 mentions) β actin, by GeneTex (1 mentions) Nerve growth factor (ngf), by GeneTex (1 mentions)

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ON-TARGETplus (235 citations) Short interfering RNAs (2 citations)

2 protocols using on targetplus short interfering si rnas

1

Cellular Signaling Pathway Analysis

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NGF (SC-365944), LOX (SC-373995), p85 (SC-1637), and Akt (SC-5298) antibodies were purchased from Santa Cruz (Biotechnology, CA, USA). The total mTOR (2983S) and phosphorylated forms of p85 (4228S), Akt (4060S), and mTOR (5536S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). β-Actin (a5441) antibody was purchased from Sigma (Cambridge, MA, USA). All ON-TARGETplus short interfering (si)RNAs were obtained from Dharmacon (Lafayette, CO, USA). Quantitative polymerase chain reaction (qPCR) primers and probes, as well as Taqman® One-Step PCR Master Mix, were supplied by Applied Biosystems (Foster City, CA, USA). Recombinant human NGF was obtained from PerpoTech (Rocky Hill, NJ, USA). All other chemicals used in this study were supplied by Sigma-Aldrich (St. Louis, MO, USA).
Tzeng H.E., Lin S.L., Thadevoos L.A., Lien M.Y., Yang W.H., Ko C.Y., Lin C.Y., Huang Y.W., Liu J.F., Fong Y.C., Chen H.T, & Tang C.H. (2021). Nerve growth factor promotes lysyl oxidase-dependent chondrosarcoma cell metastasis by suppressing miR-149-5p synthesis. Cell Death & Disease, 12(12), 1101.
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2

Signaling Pathway Antibody Optimization

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NGF, LOX, p85, Akt, mTOR and β-actin antibodies were purchased from GeneTex International Corporation (Hsinchu City, Taiwan). The phosphorylated forms of p85, Akt and mTOR antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). All ON-TARGETplus short interfering (si)RNAs were obtained from Dharmacon (Lafayette, CO, USA). Quantitative polymerase chain reaction (qPCR) primers and probes, as well as Taqman ® One-Step PCR Master Mix, were supplied by Applied Biosystems (Foster City, CA, USA). Recombinant human NGF was obtained from PerpoTech (Rocky Hill, NJ, USA). All other chemicals used in this study were supplied by Sigma-Aldrich (St. Louis, MO, USA).
Tzeng H., Lin S., Thadevoos L.A., Lien M., Yang W., Ko C., Lin C., Huang Y., Liu J., Fong Y., Chen H., & Tang C. (2021). Nerve Growth Factor Promotes Lysyl Oxidase-dependent Chondrosarcoma Cell Metastasis by Suppressing miR-149-5p Synthesis.
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