For the icv injection of ACT462206, ghrelin, and saline, a stainless steel injector (300-µm, outer diameter) was introduced through the cannula at a depth of 1.0 mm beyond the end of the guide cannula. The total volume of the solutions of ACT462206 (or saline) and ghrelin (or saline) injected into the lateral ventricle was 5 µL. A novel DORA, ACT462206, [(2S)-N-(3,5-dimethylphenyl)-1-[(4-methoxyphenyl)sulfonyl]-2-pyrrolidinecarboxamide] was purchased from TOCRIS Bioscience (Bristol, UK). Rat ghrelin was purchased from the Peptide Institute (Minoh, Japan). ACT462206 and ghrelin were dissolved in sterile 0.9% saline.
So M., Hashimoto H., Saito R., Yamamoto Y., Motojima Y., Ueno H., Sonoda S., Yoshimura M., Maruyama T., Kusuhara K, & Ueta Y. (2017). Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist. The Journal of Physiological Sciences, 68(2), 129-136.
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