At the jointing-booting stage, the second internode stems of DPW and HPW treated with different photoperiods, under growing seasons and sprayed with phytohormones were fixed in Carnoy's fixative I (3 parts of 95% ethanol to 1 part of glacial acetic acid). Sections (cross and longitudinal) with 10-μm-thick were cut on a cryostat microtome (Leica, CM1850, UV) with 1% agarose gel. After removing agarose gel using QIAquick Gel Extraction Kit (Qiagen, Cat. #: 28706, USA), the sections were stained with 1% safranin O (Solarbio) in water for 3 hours, rinsed three times in 85% ethanol for 10 minutes, and then counterstained with an alcoholic solution of fast green FCF (Solarbio, Cat. #: 907A035) (1 mg fast green FCF in 200 ml 50% ethanol) for 20 seconds. Images were captured using a Photometrics SenSys CCD camera on an Olympus BX-51 microscope (Olympus, Tokyo, Japan).
Sensys ccd camera
Manufactured by Olympus
Sourced in Japan
The SenSys CCD camera is a high-performance imaging device designed for laboratory and scientific applications. It features a charge-coupled device (CCD) sensor that captures high-quality digital images. The camera provides reliable and accurate data capture for a variety of research and analysis tasks.
Automatically generated - may contain errors
2 protocols using sensys ccd camera
1
Microscopic Analysis of Internode Development
2
Alien Chromosome Detection via GISH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic in situ hybridization (GISH) analysis was conducted to detect the alien chromosome in GN05. Seeds were germinated on moistened filter paper in Petri dishes then germinated in a constant temperature incubator at 23°C. When the roots were 2–3 cm long, roots were placed in ice water (0–4°C) for 24 h, then fixed in Carnoy’s fixative fluid (ethanol:acetic acid = 3:1) at 4°C for 2 days. The root tips were removed and digested in 1% pectinase and 2% cellulase at 37°C for 1 h, then stained with 1% (w/v) aceto-carmine solution for 2 h and squashed in 45% (v/v) acetic acid. Young spikes were stripped and fixed in 6:3:1 ethanol: chloroform:acetic acid mixture for 2 days. The anthers were squashed on a slide in 1% aceto-carmine solution. The GISH procedure was performed as described in Wang et al. (2016) . The images captured were viewed and photographed with a Photometrics SenSys CCD camera (BX61, Olympus, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!