T9988

MR experiments were conducted on a 7 T, 210 mm horizontal bore, preclinical scanner (BioSpec 70/20 USR, Bruker BioSpin MRI GmbH, Ettlingen, Germany), equipped with a gradient system of 440 mT/m at 100 μs ramp time. A quadrature volume resonator (inner diameter 35 mm, T9988; Bruker BioSpin) was used for RF excitation and signal reception. MRI data was acquired with ParaVision 5.1 software (Bruker BioSpin). Three dimensional T₂ weighted (T2W) images of the mouse whole brain were acquired with relaxation enhancement (RARE) sequence (Fig 1B). The acquisition parameters were as follows (based on the protocol TurboRARE-3D; Bruker BioSpin): repetition time (TR), 2000 ms; echo time (TE), 9 ms; effective TE, 45 ms; RARE factor, 16; acquisition matrix size, 196 × 144 × 144; field of view (FOV), 19.6×14.4×14.4 mm³; acquisition bandwidth, 75 kHz; axial orientation (coronal orientation in scanner setting): fat suppression with 2.6 ms-gaussian-shaped π/2 pulse with 1051 Hz bandwidth followed by spoiler gradient; 2 dummy scans; number of averages, 3; acquisition time, 2 h 42 m. 2.59-ms excitation and 1.94-ms refocusing pulses were used. The shape of their pulses was sinc-3 shape multiplied by a gauss function with a 25% truncation level (sinc3) and their bandwidth was 2400 Hz.

This study measured 3D T-weighted (T2W) images of the whole brain in each mouse. For this purpose, a 7T, 210-mm horizontal bore, preclinical scanner (BioSpec 70/20 USR, Bruker BioSpin MRI GmbH) MR system equipped with a 440 mT/m, 100-μs ramp time gradient system was used for relaxation enhancement (RARE) sequence was used; for RF excitation and signal reception, an orthogonal volume resonator [35 mm of inner diameter (i.d.), T9988; Bruker BioSpin] was used.
We also used a protocol called TurboRARE-3D (Bruker BioSpin), and the specific acquisition parameters are as follows: repetition time (TR) 2000 ms; echo time (TE) 9 ms; effective TE 45 ms; RARE factor 16; acquisition matrix size 196 × 144 × 144; field of view (FOV) 19.6 × 14.4 × 14.4 mm; acquisition bandwidth 75 kHz, axial (coronal direction in scanner setting): bandwidth 2.6-ms Gaussian π/2 pulse for fat suppression and spoiler gradient with 1051-Hz bandwidth, two dummy scans, averaging number 3, acquisition time 2 h 42 m, excitation pulse 2.59 ms, re-convergence pulse 1.94 ms, pulse shape: π/2 pulse, bandwidth 1051 Hz, fat suppression averaging number was 3. Pulse shape was sinc3, bandwidth was 2400 Hz. The software for the measurements was ParaVision 5.1. The cortical surface images were extracted from the measured MRI images using FSL. For details, see Ide et al. (2020).

This study measured 3D T-weighted (T2W) images of the whole brain in each mouse. For this purpose, a 7 T, 210 mm horizontal bore, preclinical scanner (BioSpec 70/20 USR, Bruker BioSpin MRIGmbH, Ettlingen, Germany) MR system equipped with a 440 mT/m, 100 μs ramp time gradient system was used for relaxation enhancement (RARE) sequence was used; for RF excitation and signal reception, an orthogonal volume resonator (35 mm i.d., T9988; Bruker BioSpin) was used.
We also used a protocol called TurboRARE-3D (Bruker BioSpin), and the specific acquisition parameters are as follows. : Repetition time (TR) 2000 ms; Echo time (TE) 9 ms; Effective TE 45 ms; RARE factor 16; Acquisition matrix size 196 × 144 × 144; Field of view (FOV) 19.6 × 14.4 × 14.4 mm; Acquisition bandwidth 75 kHz, axial (coronal direction in scanner setting): bandwidth 2.6 ms-Gaussian π/2 pulse for fat suppression and spoiler gradient with 1051 Hz bandwidth, 2 dummy scans, averaging number 3, acquisition time 2 h 42 m, excitation pulse 2.59 ms, re-convergence pulse 1.94 ms, pulse shape: π/2 pulse, bandwidth 1051 Hz, fat suppression averaging number was 3. Pulse shape was sinc3, bandwidth was 2400 Hz.
The software for the measurements was ParaVision 5.1. The cortical surface images were extracted from the measured MRI images using FSL. For details, see Ide et al. 2020.