C fos

The primers of CpG ODN 2006, β-actin, Nfatc, c-fos, RANK, matrix metalloproteinase 9 (MMP9), and 6-carboxyfluorescein (FAM)-labeled ODN (ODN and phosphorothioate ODN) were synthesized by TaKaRa Bio (Dalian, China), and the sequences are listed in Table 1. These FAM-labeled ODNs shows green signal when excited by blue light. N-Ac-L-Leu-PEI and branched PEI25K (Sigma–Aldrich, St. Louis, USA) were provided by the School of Life Sciences, Jilin University (Jilin, China). The PrimerScript^® RT reagent kit and SYBR Green Premix Ex Taq kit were purchased from TaKaRa Bio (Dalian, China). Antibodies to Nfatc, c-fos, RANK, MMP9, β-actin, and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies were all purchased from ABclonal (Boston, MA, USA). The RAW264.7 cells (SCSP-5036) were purchased from the cell bank at the Chinese Academy of Sciences (Shanghai, China).Table 1

The Primer Sequences of CpG ODN 2006, β-Action, Nfatc, c-fos, RANK and MMP9

Name	Sequence
2006	5ʹ-TCGTCGTTTTGTCGTTTTGTCGTT-3’
β-actin	5ʹ-CATCCGTAAAGACCTCTATGCCAAC-3ʹ5ʹ-ATGGAGCCACCGATCCACA-3’
Nfatc	5ʹ-CAAGTCTCACCACAGGGCTCACTA-3ʹ5ʹ-TCAGCCGTCCCAATGAACAG-3’
c-fos	5ʹ-ACGTGGAGCTGAAGGCAGAAC-3ʹ5ʹ-AGCCACTGGGCCTAGATGATG-3’
RANK	5ʹ-GGCTTACCTGCCCAGTCTCATC-3ʹ5ʹ-AAGCATCATTGACCCAATTCCAC-3’
MMP9	5ʹ-GCCCTGGAACTCACACGACA-3ʹ5ʹ-TTGGAAACTCACACGCCAGAAG-3’